To study the chromatin structure of PIND, muclei from NIH3T3 cells and human growth hormone- treated NIH3T3 cells are incubated with DNAseI. Next, the DNA is separated by size on an agarose gel. 4. Explain how DNASE I cuts chromatin. A drawing is welcome. C hGH PIND found here Figure 3.2 Agarose gel electrophoresis of chromatin DNA from NIH3T3 cells incubated with DNAseI (C) and chromatin DNA PIND found here from human growth hormone-treated NIH3T3 cells incubated with DNAsel (hGH). The area of the gel where PIND gene sequences were found are indicated with red boxes. The DNA is purified from the gel and sequenced (figure 3.2). This shows that the PIND gene is almost uncut by DNAS I in NIH3T3 cells but is cut into small pieces in the cells treated with DNAsel 5. What does this experiment tell about the effect of human growth hormone on the chromatin structure of the PIND gene?

To study the chromatin structure of PIND, muclei from NIH3T3 cells and human growth hormone- treated NIH3T3 cells are incubated with DNAseI. Next, the DNA is separated by size on an agarose gel. 4. Explain how DNASE I cuts chromatin. A drawing is welcome. C hGH PIND found here Figure 3.2 Agarose gel electrophoresis of chromatin DNA from NIH3T3 cells incubated with DNAseI (C) and chromatin DNA PIND found here from human growth hormone-treated NIH3T3 cells incubated with DNAsel (hGH). The area of the gel where PIND gene sequences were found are indicated with red boxes. The DNA is purified from the gel and sequenced (figure 3.2). This shows that the PIND gene is almost uncut by DNAS I in NIH3T3 cells but is cut into small pieces in the cells treated with DNAsel 5. What does this experiment tell about the effect of human growth hormone on the chromatin structure of the PIND gene?

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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