To study the chromatin structure of PIND, muclei from NIH3T3 cells and human growth hormone- treated NIH3T3 cells are incubated with DNAseI. Next, the DNA is separated by size on an agarose gel. 4. Explain how DNASE I cuts chromatin. A drawing is welcome. C hGH PIND found here Figure 3.2 Agarose gel electrophoresis of chromatin DNA from NIH3T3 cells incubated with DNAseI (C) and chromatin DNA PIND found here from human growth hormone-treated NIH3T3 cells incubated with DNAsel (hGH). The area of the gel where PIND gene sequences were found are indicated with red boxes. The DNA is purified from the gel and sequenced (figure 3.2). This shows that the PIND gene is almost uncut by DNAS I in NIH3T3 cells but is cut into small pieces in the cells treated with DNAsel 5. What does this experiment tell about the effect of human growth hormone on the chromatin structure of the PIND gene?
To study the chromatin structure of PIND, muclei from NIH3T3 cells and human growth hormone- treated NIH3T3 cells are incubated with DNAseI. Next, the DNA is separated by size on an agarose gel. 4. Explain how DNASE I cuts chromatin. A drawing is welcome. C hGH PIND found here Figure 3.2 Agarose gel electrophoresis of chromatin DNA from NIH3T3 cells incubated with DNAseI (C) and chromatin DNA PIND found here from human growth hormone-treated NIH3T3 cells incubated with DNAsel (hGH). The area of the gel where PIND gene sequences were found are indicated with red boxes. The DNA is purified from the gel and sequenced (figure 3.2). This shows that the PIND gene is almost uncut by DNAS I in NIH3T3 cells but is cut into small pieces in the cells treated with DNAsel 5. What does this experiment tell about the effect of human growth hormone on the chromatin structure of the PIND gene?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Transcribed Image Text:To study the chromatin structure of PIND, nuclei from NIH3T3 cells and human growth hormone-
treated NIH3T3 cells are incubated with DNAsel. Next, the DNA is separated by size on an agarose
gel.
4. Explain how DNASE I cuts chromatin. A drawing is welcome.
C hGH
PIND found here
Figure 3.2 Agarose gel electrophoresis of chromatin DNA from
NIH3T3 cells incubated with DNAsel (C) and chromatin DNA
from human growth hormone-treated NIH3T3 cells incubated with
DNAsel (hGH).
PIND found here
The area of the gel where PIND gene sequences were found are
indicated with red boxes.
The DNA is purified from the gel and sequenced (figure 3.2). This shows that the PIND gene is
almost uncut by DNAS I in NIH3T3 cells but is cut into small pieces in the cells treated with
DNAsel
5. What does this experiment tell about the effect of human growth hormone on the
chromatin structure of the PIND gene?
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