A purified protein is in a Hepes (N-(2-hydroxy-ethyl)piperazine-N′-(2-ethanesulfonic acid)) buffer at pH 7 with 500 mM NaCl. A sample (1 mL) of the protein solution is placed in a tube made of dialysis membrane and dialyzed against 1 L of the same Hepes buffer with 0 mM NaCl. Small molecules and ions (such as Na+, Cl+, and Hepes) can diffuse across the dialysis membrane, but the protein cannot.(a) Once the dialysis has come to equilibrium, what is the concentration of NaCl in the protein sample? Assume no volume changes occur in the sample during the dialysis.(b) If the original 1 mL sample were dialyzed twice, successively, against 100 mL of the same Hepes buffer with 0 mM NaCl, what would be the final NaCl concentration in the sample?
Nucleotides
It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic Acids
Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.
A purified protein is in a Hepes (N-(2-hydroxy-ethyl)piperazine-N′-(2-
ethanesulfonic acid)) buffer at pH 7 with 500 mM NaCl. A sample (1 mL) of the protein solution is placed in a tube made of dialysis membrane and dialyzed against 1 L of the same Hepes buffer with 0 mM NaCl. Small molecules and ions (such as Na+, Cl+, and Hepes) can diffuse across the dialysis membrane, but the protein cannot.
(a) Once the dialysis has come to equilibrium, what is the concentration of NaCl in the protein sample? Assume no volume changes occur in the sample during the dialysis.
(b) If the original 1 mL sample were dialyzed twice, successively, against 100 mL of the same Hepes buffer with 0 mM NaCl, what would be the final NaCl concentration in the sample?
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