Which of the following mutations is most likely to cause a loss of protein function? O a. Silent mutation: (RNA codes for the same amino acid.) O b. Nonsense mutation: (RNA codes for an early stop codon.) O c. Missense mutation: (RNA codes for a different amino acid.) O d. Frameshift mutation: (Base pairs are added or removed.) You need to fill an agarose gel tank with about 300 mL of TAE buffer. Unfortunately, one of your labmates was inconsiderate and used up all of the 1X TAE buffer without making more. Fortunately, you have found a bottle of 50X TAE stock solution. How much of the 50X stock solution should you combine with how much water to make 300 mL of 1X TAE? Remember to include units in your answer. Preparing 1 L (liter) of bacterial growth medium involves dissolving 25 g of pre-mixed LB powder in a final volume of 1 L of water. If you need to prepare 700 mL of medium, how many grams of LB do you need to weigh out?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
Question

Need help, please.

 

Which of the following mutations is most likely to cause a loss of protein function?
a. Silent mutation: (RNA codes for the same amino acid.)
b. Nonsense mutation: (RNA codes for an early stop codon.)
c. Missense mutation: (RNA codes for a different amino acid.)
d. Frameshift mutation: (Base pairs are added or removed.)
You need to fill an agarose gel tank with about 300 mL of TAE buffer. Unfortunately, one of your labmates was inconsiderate and used up all of the 1X TAE buffer without making
more. Fortunately, you have found a bottle of 50X TAE stock solution. How much of the 50X stock solution should you combine with how much water to make 300 mL of 1X
TAE? Remember to include units in your answer.
Preparing 1 L (liter) of bacterial growth medium involves dissolving 25 g of pre-mixed LB powder in a final volume of 1 L of water. If you need to prepare 700 mL of medium, how
many grams of LB do you need to weigh out?
Transcribed Image Text:Which of the following mutations is most likely to cause a loss of protein function? a. Silent mutation: (RNA codes for the same amino acid.) b. Nonsense mutation: (RNA codes for an early stop codon.) c. Missense mutation: (RNA codes for a different amino acid.) d. Frameshift mutation: (Base pairs are added or removed.) You need to fill an agarose gel tank with about 300 mL of TAE buffer. Unfortunately, one of your labmates was inconsiderate and used up all of the 1X TAE buffer without making more. Fortunately, you have found a bottle of 50X TAE stock solution. How much of the 50X stock solution should you combine with how much water to make 300 mL of 1X TAE? Remember to include units in your answer. Preparing 1 L (liter) of bacterial growth medium involves dissolving 25 g of pre-mixed LB powder in a final volume of 1 L of water. If you need to prepare 700 mL of medium, how many grams of LB do you need to weigh out?
Expert Solution
steps

Step by step

Solved in 4 steps

Blurred answer
Similar questions
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON