Which of the following mutations is most likely to cause a loss of protein function? O a. Silent mutation: (RNA codes for the same amino acid.) O b. Nonsense mutation: (RNA codes for an early stop codon.) O c. Missense mutation: (RNA codes for a different amino acid.) O d. Frameshift mutation: (Base pairs are added or removed.) You need to fill an agarose gel tank with about 300 mL of TAE buffer. Unfortunately, one of your labmates was inconsiderate and used up all of the 1X TAE buffer without making more. Fortunately, you have found a bottle of 50X TAE stock solution. How much of the 50X stock solution should you combine with how much water to make 300 mL of 1X TAE? Remember to include units in your answer. Preparing 1 L (liter) of bacterial growth medium involves dissolving 25 g of pre-mixed LB powder in a final volume of 1 L of water. If you need to prepare 700 mL of medium, how many grams of LB do you need to weigh out?
Which of the following mutations is most likely to cause a loss of protein function? O a. Silent mutation: (RNA codes for the same amino acid.) O b. Nonsense mutation: (RNA codes for an early stop codon.) O c. Missense mutation: (RNA codes for a different amino acid.) O d. Frameshift mutation: (Base pairs are added or removed.) You need to fill an agarose gel tank with about 300 mL of TAE buffer. Unfortunately, one of your labmates was inconsiderate and used up all of the 1X TAE buffer without making more. Fortunately, you have found a bottle of 50X TAE stock solution. How much of the 50X stock solution should you combine with how much water to make 300 mL of 1X TAE? Remember to include units in your answer. Preparing 1 L (liter) of bacterial growth medium involves dissolving 25 g of pre-mixed LB powder in a final volume of 1 L of water. If you need to prepare 700 mL of medium, how many grams of LB do you need to weigh out?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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