5a. The table below summarizes expression of B-galactosidase (lacZ) and permease (lacY) from the lac operon, respectively. The strains are grown in the absence or presence of lactose. The “_“ indicates no/low expression and “+" indicates high expression. For each strain (A-G) write out one possible genotype (more than one might be possible) in the table for a lac operon that is consistent with the expression pattern. Do not give partial diploid genotypes. The first condition (upper right) is filled in for you as an example. Strain Lactose Absent Lactose Present B-galactosidase Permease B-galactosidase Permease Lac operon genotype(s) а. + lac+lacP+lacO+lacZ+lacY+ b. d. e. f. + + + g. +|+
5a. The table below summarizes expression of B-galactosidase (lacZ) and permease (lacY) from the lac operon, respectively. The strains are grown in the absence or presence of lactose. The “_“ indicates no/low expression and “+" indicates high expression. For each strain (A-G) write out one possible genotype (more than one might be possible) in the table for a lac operon that is consistent with the expression pattern. Do not give partial diploid genotypes. The first condition (upper right) is filled in for you as an example. Strain Lactose Absent Lactose Present B-galactosidase Permease B-galactosidase Permease Lac operon genotype(s) а. + lac+lacP+lacO+lacZ+lacY+ b. d. e. f. + + + g. +|+
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:5a. The table below summarizes expression of B-galactosidase (lacZ) and permease (lacY) from the lac
operon, respectively. The strains are grown in the absence or presence of lactose. The "_“ indicates no/low
expression and “+" indicates high expression. For each strain (A-G) write out one possible genotype
(more than one might be possible) in the table for a lac operon that is consistent with the expression
pattern. Do not give partial diploid genotypes. The first condition (upper right) is filled in for you as an
example.
Strain
Lactose Absent
Lactose Present
B-galactosidase Permease B-galactosidase
Permease
Lac operon genotype(s)
а.
+
+
lac+lacP+lacO+lacZ+lacY+
b.
d.
e.
f.
+
+
+
+
g.
+
4
5b. Assuming that the lac operon genotype in strain e contains a trans-acting mutation, would adding a
copy of lact on a plasmid be dominant or recessive to the existing mutation? Explain.
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