Comparison of the genomes of wild-type bovine tuberculosis strains of Mycobacterium bovis and contemporary M. bovis-derived Bacille Calmette-Guerin (BCG) vaccine strains with that of a virulent reference strain of M. tuberculosis indicated that almost 100 M. tuberculosis genes were found to be “missing” from all of the M. bovis and BCG isolates that were used for making making the vaccines. Describe how you might isolate and identify those genes that are unique to M. tuberculosis.

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