A) What additional reagents would you need to perform immunostaining of frozen sections of mouse thymus tissue for Keratin 8 that is expressed in cortical thymic epithelial cells and Keratin 5 that is expressed in mouse medullary thymic epithelial cells? B) Create a staining protocol including all tubes and appropriate controls to stain 4 slides with frozen thymic sections. You will need 200ul of antibody staining solution for each slide.
A) What additional reagents would you need to perform immunostaining of frozen sections of mouse thymus tissue for Keratin 8 that is expressed in cortical thymic epithelial cells and Keratin 5 that is expressed in mouse medullary thymic epithelial cells? B) Create a staining protocol including all tubes and appropriate controls to stain 4 slides with frozen thymic sections. You will need 200ul of antibody staining solution for each slide.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You have the following reagents available Rat anti-Keratin 8 antibody specific for mouse Keratin 8, Rabbit polyclonal antibody against mouse keratin 5 and antifade reagent with DAPI to stain DNA. Both primary antibodies work best at 1:500 dilution. A) What additional reagents would you need to perform immunostaining of frozen sections of mouse thymus tissue for Keratin 8 that is expressed in cortical thymic epithelial cells and Keratin 5 that is expressed in mouse medullary thymic epithelial cells? B) Create a staining protocol including all tubes and appropriate controls to stain 4 slides with frozen thymic sections. You will need 200ul of antibody staining solution for each slide.
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