You are interested in performing indirectimmunofluorescence light microscopy toobserve the localization of the catalaseenzyme in the cultured HeLa cells,obtained historically from the cervicaltumor of Henrietta Lacks. You were goingthrough the lab stock and found a fewprimary and secondary antibodies. Whichof the following secondary antibody canyou use in your experiments?O All of the mentioned antibodies can beused in the experimentGoat anti-human antibody conjugated to10 nm goldGoat-anti-human catalase conjugated to 10nm goldO Human anti-catalase antibody conjugatedto fluorescent rhodamineGoat anti-human antibody conjugated tofluorescent rhodamine

Answered: You are interested in performing…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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You are interested in performing indirect
immunofluorescence light microscopy to
observe the localization of the catalase
enzyme in the cultured HeLa cells,
obtained historically from the cervical
tumor of Henrietta Lacks. You were going
through the lab stock and found a few
primary and secondary antibodies. Which
of the following secondary antibody can
you use in your experiments?
O All of the mentioned antibodies can be
used in the experiment
Goat anti-human antibody conjugated to
10 nm gold
Goat-anti-human catalase conjugated to 10
nm gold
O Human anti-catalase antibody conjugated
to fluorescent rhodamine
Goat anti-human antibody conjugated to
fluorescent rhodamine

Expert Solution

Step 1: Introduction

Immunofluorescence light microscopy is a laboratory technique that uses fluorescently labeled antibodies to visualize specific proteins or molecules within cells or tissues. It involves binding a primary antibody to the target molecule and then using a secondary antibody labeled with a fluorescent dye to detect and visualize the location and distribution of the target in a sample under a fluorescence microscope. This method is widely used in cell biology and pathology to study cellular structures, protein localization, and disease markers with high specificity and sensitivity.

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