Describe a series of experiments to SELECT and/or SCREEN for strains containing mutations that specifically disrupt the function of StyRS/StyAB and not StyC/StyD in Pseudomonas strain Y2 .
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
There is a chemical called styrene which is used in many industries to make latex, polystyrene resins, and synthetic rubber. This chemical is very dangerous if not removed from the airborne styrene gases released by industries as it has a carcinogenic effect.
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