A donor strain of bacteria with genotype leu+ gal− pro+ is infected with phages. The phage lysate from the bacterial cells is collected and used to infect a second strain of bacteria that are leu− gal+ pro−. The second strain is selected for leu+, and the following cotransduction data are obtained: Donor Recipient Selected allele Cells with cotransduced allele (%) leu+ gal− pro+ leu− gal+ pro− leu+ 47 pro+ leu+ 26 gal− Which genes are closest, leu and gal or leu and pro?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
A donor strain of bacteria with genotype leu+ gal− pro+ is infected
with phages. The phage lysate from the bacterial cells is collected
and used to infect a second strain of bacteria that are leu− gal+ pro−.
The second strain is selected for leu+, and the following
cotransduction data are obtained:
Donor Recipient Selected
allele
Cells with
cotransduced
allele (%)
leu+
gal−
pro+
leu− gal+
pro− leu+ 47 pro+
leu+ 26 gal−
Which genes are closest, leu and gal or leu and pro?
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