Scientists at the SEA Phages lab were asked to find a phage that might infect a strain of M. abscessus that is infecting a young patient. Because the scientists did not have any phages known to infect M. abscessus, they first tested M. smeg phages that are also known to infect M. tuberculosis to see if they could infect this specific strain of M. abscessus. Why did they start with those phages? Select the best answer. O Phages that infect M. tuberculosis will also infect M. abscessus O The phages have already been FDA approved for use in phage therapy O Phages that infect two different hosts have a broader host range and are more likely to be able to infect a third host (such as M. abcessus) O The phages are lytic, which makes them good candidates for phage therapy O More information is known about the genomes of these phages than other phages

Q: Why is it easier to target a single mutant versus multiple mutants in the virus?

A: A virus is an infection-causing microbial agent that replicates inside a living cell of an organism.…

Q: The figure below shows a partial chromosome map of an E. coli Hfr strain.  Each mark = 10 minutes…

A: Given: A partial chromosome map of an E. coli Hfr strain.  Each mark = 10 minutes between…

Q: What method is used by bacteriophages to release from the infected host cell? O 1) budding O 2)…

A: Answer is option 4.)lysis.

Q: Describe the reason behind the occurrence of phage growth and release by the mating with the…

A: Bacteriophages frequently transition between the lytic and lysogenic states. The two gene regulatory…

Q: In Hershey-Chase experiment, bacteriophages protein coats were tagged with radioactive isotope S-32.…

A: Hershey and Chase's experiment was based on the principle to identify DNA as genetic material. they…

Q: If a single bacteriophage infects one E. coli cell present on a lawn of bacteria and, upon lysis,…

A: Bacteria are microscopic, single-celled organic entities that thrive in diverse environments. These…

Q: Are the normal and pathogenic strains of E.coli separate species

A: Escherichia coli is Gram-negative bacilli that are normally found in the intestines and the gut of…

Q: You are designing a phage therapy for a cystic fibrosis patient with an multi-antibiotic resistant…

A: The most crucial characteristic for this particular phage therapy scenario is: C. That the infection…

Q: triphosphates (dNTPs) or nucleoside triphosphates (NTPS).

A: Viruses are not capable of translating the proteins,  thus they are dependent on host machinery for…

Q: Which type of phages can perform   specialized  transduction?

A: Transduction is the process by which foreign DNA is introduced into the cell by a suitable virus or…

Q: Austin Taylor and Edward Adelberg isolated some 200 new strains of Hfr cells that they then used to…

A: The transformation, transduction, and conjugation are the horizontal transfer mechanism through…

Q: Which type of phages can perform   generalized transduction?

A: Zinder and Lederberg in 1952 discovered Transduction in S.typhimurum. The phage which mediated DNA…

Q: Using phages that came from E. coli with the genotype al+ be+, you infect a strain ofE. coli that is…

A: Auxotrophs are the term used for the organism or bacteria that cannot express a gene due to mutation…

Q: Compare and contrast the lysogenic and lytic cycles of phage λ.

A: Phage lambda is a bacteriophage which infects E.coli bacteria. Bacteriophages are the viruses that…

Q: How did clone USA300 and other strains of MRSA become so dangerous?

A: The reason is;

Q: briefly describe four explanations why E. coli phages can only infect E. coli cells, but not other…

A: Question - briefly describe four explanation why E.coli phage can only infect E.coli cells, but not…

Q: There are plenty of different phages that will kill Pseudomonas aeruginosa bacteria.  Why did the…

A: Note:- Sorry, as per the honor code, referring to external citations is not allowed. However, we…

Q: You are given two strains of E. coli. The Hfr strain is arg+ ala+ glu+ pro+ leu+ T s; the F− strain…

A: F- Strain susceptible to Pen but ltfr strain resistant So, ltfr strain must be the time it has per r…

Q: Which of the following is a way in which plasmids may be introduced into host organisms?

A: Plasmids or transposons are considered replicons, units of DNA capable of replicating autonomously…

Q: What is required to package the T4 genome into its phage head?

A: Introduction  T4 is a type bacteriophage which infects the E coli bacteria (Escherichia coli). It is…

Q: osaic disease was NOT caused by a bacteria?

A: The virus can be defined as the submicroscopic infectious agent that can replicate inside the living…

Q: Which of the following statements describes the lysogenic cycle of lambda (A) phage? After…

A: In this lambda phage, the virus infects the bacteria, this virus can perform two types of life…

Q: Why is it important to kill all the bacteria in the raw sewage during phage isolation?

A: Bacteriophage for a specific bacterium can be isolated from any environment where that bacterium is…

Q: Which of the folowing statements best describes future generations of these bacteria? A) They will…

A: Antibiotic is used against the bacterial infection, and to suppresses the growth of the bacterial…

Q: Please give reasons why  bacteriophage would not infect a bacteria

A: Bacteria are unicellular microscopic organisms that live in wide variety of environment. Bacteria…

Q: 150 100 xyl+ mtl+ 50 met+ 20 40 60 80 100

A:

Q: S. aureus is gram positive. What differences in strains might account for differences in phage…

A: Bacteria are microscopic single-celled organisms that thrive in diverse environmental conditions.…

Q: During the lysogenic cycle of lambda (A) phage the phage genome is integrated in the host chromosome…

A: Bacteriophage are those viruses which are found to be in fact to the bacterial cell.

Q: A phage that reproduces slowly, produces relatively few phage particles, and only undergoes the…

A:

Q: Most temperate phages integrate into the host chromosome, whereas some replicate as plasmids. Which…

A: Temperate phages are those phages that exhibit a lysogenic life cycle. The lysogenic cycle refers to…

Q: ne mL of a bacteriophage suspension is mixed with 20 mL of a bacterial culture and 50% of the phages…

A: A bacteriophage is mostly made up of a nucleic acid molecule that is enclosed by a protein…

Q: Which of the following is NOT a feature of genetic reassortment? A)  a host cell is coinfected with…

A: Genetic reassortment is the changes in the genome of the virus that affect the host. This helps in…

Q: How many bacteriophage particles were isolated from a single plaque? Howmany different strains of…

A: Bacteriophages, which are mainly infected bacteria are going through the lytic or the lysogenic…

Q: Which of the following statements regarding transduction is FALSE? A) transduction reduces genetic…

A: A virus moves its genetic information from one bacterium to another through a process called…

Q: E. coli

A: Bacterial Strain LE392 is used for both genomic and cDNA cloning. LE392 lacks the E. coli K…

Q: The contents of a plaque may not always contain a clonal population of phage if: a. Identical…

A: Plaque:- phage infected bacterium usually burst and releases a number of daughter phages which…

Q: After sequencing E.coli ROAR340 strain and using Silico Clermont Phylotyper tools show that is it…

A: Escherichia coli, also known as E. coli, is a gram-negative, facultative anaerobic, rod-shaped…

Q: From what other environments might E. coli bacteriophages be readily isolated?

A: Some of the prokaryotes, especially bacteria are infected with the viruses. These viruses are known…

Q: How are people who lack health insurance or are underinsured affected by the COVID-19 pandemic in…

A: People who lack health insurance or are underinsured are significantly affected by the COVID-19…

Q: Austin Taylor and Edward Adelberg isolated some new strains of Hfr cells that they then used to map…

A: Transfer of genes in prokaryotes occurs through horizontal transfer mechanisms such as transduction,…

Q: In 2013, there was an outbreak of methicillin-resistant Staphylococcusaureus (MRSA) at an NFL…

A: The study of what makes a certain action in a specific scenario the proper thing to do is known as…

Q: A bacteriophage λ is found that is able to lysogenize itsE. coli host at 30°C but not at 42°C. What…

A: Introduction  Viruses have two types of life cycles either the Lytic cycle or the Lysogenic life…

Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.