Describe what each of the 12 bands on your Western blot should have been. Remember, the 12 bands will be 4 conditions x 3 proteins (phospho-S6, phospho-AMPK, tubulin). Please describe the relative density of each band compared to the control (for example, how dense will phospho-S6 be in each of the three experimental conditions compared to the control condition?). For each band, provide a well-reasoned rationale for your anticipated result. Give the reason why cell signaling would produce each result in each condition.

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter16: Adult And Pediatric Dosages Based On Body Surface Area
Section: Chapter Questions
Problem 3.3P
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Describe what each of the 12 bands on your Western blot should have beenRemember, the 12 bands will be 4 conditions x 3 proteins (phospho-S6, phospho-AMPK, tubulin). Please describe the relative density of each band compared to the control (for example, how dense will phospho-S6 be in each of the three experimental conditions compared to the control condition?). For each band, provide a well-reasoned rationale for your anticipated result. Give the reason why cell signaling would produce each result in each condition.

Formulation 1
5 mM
2 mM
Glucose
Glutamine
Pyruvate
1 mM
FBS
10%
Formulation 2
Glucose
25 mM
Glutamine
2 mM
Pyruvate
1 mM
FBS
10%
Formulation 3
Glucose
10 mM
Glutamine
2 mM
Pyruvate
1 mM
FBS
10%
Formulation 4
Glucose
1 mM
Glutamine
2 mM
Pyruvate
1 mM
FBS
10%
Transcribed Image Text:Formulation 1 5 mM 2 mM Glucose Glutamine Pyruvate 1 mM FBS 10% Formulation 2 Glucose 25 mM Glutamine 2 mM Pyruvate 1 mM FBS 10% Formulation 3 Glucose 10 mM Glutamine 2 mM Pyruvate 1 mM FBS 10% Formulation 4 Glucose 1 mM Glutamine 2 mM Pyruvate 1 mM FBS 10%
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