Describe what each of the 12 bands on your Western blot should have been. Remember, the 12 bands will be 4 conditions x 3 proteins (phospho-S6, phospho-AMPK, tubulin). Please describe the relative density of each band compared to the control (for example, how dense will phospho-S6 be in each of the three experimental conditions compared to the control condition?). For each band, provide a well-reasoned rationale for your anticipated result. Give the reason why cell signaling would produce each result in each condition.

Q: Describe the banding pattern seen in the gel. What does that tell you about the role of annealing…

A: Gel electrophoresis is a laboratory technique for separating charged molecules such as DNA, RNA, and…

Q: An experiment was performed in mice.  Wild-type bone marrow is used to reconstitute lethally…

A: The immune system is the body’s defense mechanism against infections and invading pathogens and is…

Q: Among the following statements regarding the use of viral vectors, which is (are) true: (can be more…

A: Transfer of foreign nucleic acid insert into the eukaryotic cell is transfection. For many…

Q: A chain of biochemical events is responsible for Aequorea victoria turning green. Firstly, the…

A: Gel filtration chromatography, or size exclusion chromatography, is a chromatographic technique for…

Q: Looking at the sequence of gene X, you hypothesize that there is an intrinsic terminator in the 3'…

A: Blot A shows a band for both the wild-type and IT gene, indicating that the ΔIT gene is still…

Q: Explain

A: The question is asking to calculate the melting temperature (Tm) and the percentage of…

Q: I know the answer is e but can you thoroughly explain the answer choices? like what does…

A: The prokaryotic gene regulation is known as operon system which regulates the transcription of a…

Q: MDF57 is a 110kDa DNA binding protein which has some functional roles in the development of…

A: Mutation is any change in the genetic setup of the cell such as change in the sequence, structure or…

Q: What sort of selection marker system will ensure that only E. coli transformed with pGLO will…

A: pGLO is a plasmid that is frequently used in genetic engineering experiments. It contains a number…

Q: If your analysis identifies that the total Cytosine content in a sample is 29%, what will be the…

A: According to Chargaff's rule number of purine(adenine+guanine) = number of pyrimidine (cytosine +…

Q: You run a Western blot to detect Ras in sampe A and sample B. You find that sample B gives a darker…

A: First of all, Western Blot is a technique which is used to detect the expression of protein from…

Q: Determine the size of the DNA you would expect from the restriction digests of the GABRD and GABRD…

A: Gel electrophoresis is a technique used to separate DNA, RNA, or protein molecules based on their…

Q: In the absence of tryptophan and the trpD structural gene contains a frameshift insertion mutation,…

A:

Q: quence is an intrinsic terminator). wild-type ΔΙΤ A Blot B Blot D Blot E Blot A Blot C wild-type ΔΙΤ…

A: The above image is of Northern blotting which shows separation of RNA on the gel. The gel used in…

Q: Gene Actin (housekeeping gene) Annexin I c. You hybridize the cDNA to your microarrays, scan your…

A: cDNA stands for "complementary DNA" and it is a single-stranded DNA molecule that is synthesized…

Q: Continuing from the question above. You are able to correctly modify the blood stem cells and return…

A: Sickle cell anemia is majorly due to missense mutation which results in valine substitution in place…

Q: A fluctuation test was carried out to determine the rate of mutation to Azetidine resistance (a…

A: The inventors of fluctuation test were Delburk and Luria in 1943. This test aimed to determine…

Q: The carboxy terminus of the p53 protein acts as an allosteric regulator of sequence-specific DNA…

A: General structure of p53 protein

Q: What technique would you use for each senario(a and b) and why. FISH, immunofluorescence, and GFP…

A: The use of cytogenetic analysis as part of diagnostic procedures for various human conditions is…

Q: 1) In MALDI-TOF data, when performing peptide mass mapping search, a) The number of peptides…

A: Protein identification with the help of mass spectrometry (MS) is an major step in proteomic…

Q: Knock out mice that are mutant for a gene product X die. Variants of the X gene that were…

A: The physical crossing over during meiosis of parental heterozygous homologous chromosomes or genes…

Q: was reduced in the knock-out mutant cells. The N-terminal region of Protein X is 100% conserved…

A: As per our policy we are answering the first three sub parts ( a, b , c ). Of the Question For the…

Q: A 2.0kb bacterial plasmid ‘BS1030’ is digested with the restriction endonuclease Sau3A; the plasmid…

A: Plasmids are small, circular, double stranded, and extrachromosomal bacterial DNA. i.e. it is…

Q: 28. In a transformation experiment, DNA is collected from an E. coli donor strain of genotype cys(+)…

A: There are three modes of transfer of genetic information in bacteria. They are transduction,…

Q: Several DNA coding for different proteins, CRISPR, or siRNAs against different genes were expressed…

A: The given experimental setup involves the expression of various proteins and treatments using siRNAs…

Genomic studies

Genomic studies (genomics) is the study of the whole genome of an organism. Using bioinformatics and high-performance computing, genomic researchers examined massive amounts of deoxyribonucleic acid (DNA)-sequence data to discover variations or mutations that affect drug response, health, or disease. Genomics is a much younger discipline than genetics, having only emerged in the last few decades as a result of technological advancements in DNA sequencing and computational biology.