Describe what each of the 12 bands on your Western blot should have been. Remember, the 12 bands will be 4 conditions x 3 proteins (phospho-S6, phospho-AMPK, tubulin). Please describe the relative density of each band compared to the control (for example, how dense will phospho-S6 be in each of the three experimental conditions compared to the control condition?). For each band, provide a well-reasoned rationale for your anticipated result. Give the reason why cell signaling would produce each result in each condition.

Q: You observe the following: Your Your friend's Gel Gel 120 kDa 100 kDa 120 kDa -100 kDa -80 kDa 80…

A: Blotting, as we all know, is a process that uses electrophoresis to separate DNA, RNA, and protein…

Q: Describe the banding pattern seen in the gel. What does that tell you about the role of annealing…

A: Gel electrophoresis is a laboratory technique for separating charged molecules such as DNA, RNA, and…

Q: An experiment was performed in mice.  Wild-type bone marrow is used to reconstitute lethally…

A: The immune system is the body’s defense mechanism against infections and invading pathogens and is…

Q: What is the advantage to transferring proteins bands from a gel to nitrocellulose during the western…

A: The western blot is a broadly utilized scientific procedure in molecular biology and immunogenetics…

Q: ii) In order to create a western blot, SDS-PAGE is first performed to separate the proteins. After…

A: SDS-PAGE is a protein separation procedure, most widely used in immunoblotting. It can be done once…

Q: segment shown in the diagram. The first three nucleotides of sequencing read 1 and sequencing read…

A: Nucleotides are the building blocks of DNA and RNA. They are organic molecules that consist of a…

Q: Search the NCBI protein database using the EC number, limit your search to Mycobacterium…

A: We know about protein data base as we get the information of nucleic acid sequence or protein…

Q: Among the following statements regarding the use of viral vectors, which is (are) true: (can be more…

A: Transfer of foreign nucleic acid insert into the eukaryotic cell is transfection. For many…

Q: "The antibody diversity created by the combinatorial joining of V, D, and J segments by V(D)J…

A: Early phases of T and B lymphocyte development are characterised by V(D)J recombination, which takes…

Q: A chain of biochemical events is responsible for Aequorea victoria turning green. Firstly, the…

A: Gel filtration chromatography, or size exclusion chromatography, is a chromatographic technique for…

Q: Looking at the sequence of gene X, you hypothesize that there is an intrinsic terminator in the 3'…

A: Blot A shows a band for both the wild-type and IT gene, indicating that the ΔIT gene is still…

Q: Mutation analysis of GCK gene in patients with diabetes revealed a c.114 TA (shown in bold and…

A: Restriction enzyme are the enzyme which cuts the DNA at specific recognition site.

Q: Explain

A: The question is asking to calculate the melting temperature (Tm) and the percentage of…

Q: The buffers used for the IMAC separation of His-tagged GFP method represent a typical biotech lab…

A: Immobilized metal affinity chromatography (IMAC) is a method of protein separation based on the…

Q: You are trying to determine the cell count per microliter of some melanoma cells that have been…

A: Stock solutions often have a very high concentration of cells which makes it manually impossible to…

Q: I know the answer is e but can you thoroughly explain the answer choices? like what does…

A: The prokaryotic gene regulation is known as operon system which regulates the transcription of a…

Q: MDF57 is a 110kDa DNA binding protein which has some functional roles in the development of…

A: Mutation is any change in the genetic setup of the cell such as change in the sequence, structure or…

Q: What sort of selection marker system will ensure that only E. coli transformed with pGLO will…

A: pGLO is a plasmid that is frequently used in genetic engineering experiments. It contains a number…

Q: Which classification of AML best fits this morphology, immunophenotyping, and cytochemistry? Select…

A: Leukemia is a specific type of blood cancer that is caused due to the abnormal increases in the…

Q: If your analysis identifies that the total Cytosine content in a sample is 29%, what will be the…

A: According to Chargaff's rule number of purine(adenine+guanine) = number of pyrimidine (cytosine +…

Q: You run a Western blot to detect Ras in sampe A and sample B. You find that sample B gives a darker…

A: First of all, Western Blot is a technique which is used to detect the expression of protein from…

Q: Expressing genes encoding a particular protein’s subunits from a plasmid in E. coli cells (volume of…

A: Escherichia coli (E. coli) are group of bacteria that are prevalent in the environment, foods, and…

Q: With respect to recombinant vaccines, it is CORRECT to state that: * (Only one statement is correct)…

A: With respect to recombinant vaccines, it is CORRECT to state that: (a) Protein subunit vaccines are…

Q: Determine the size of the DNA you would expect from the restriction digests of the GABRD and GABRD…

A: Gel electrophoresis is a technique used to separate DNA, RNA, or protein molecules based on their…

Q: In the absence of tryptophan and the trpD structural gene contains a frameshift insertion mutation,…

A:

Q: quence is an intrinsic terminator). wild-type ΔΙΤ A Blot B Blot D Blot E Blot A Blot C wild-type ΔΙΤ…

A: The above image is of Northern blotting which shows separation of RNA on the gel. The gel used in…

Q: Gene Actin (housekeeping gene) Annexin I c. You hybridize the cDNA to your microarrays, scan your…

A: cDNA stands for "complementary DNA" and it is a single-stranded DNA molecule that is synthesized…

Q: Continuing from the question above. You are able to correctly modify the blood stem cells and return…

A: Sickle cell anemia is majorly due to missense mutation which results in valine substitution in place…

Q: A fluctuation test was carried out to determine the rate of mutation to Azetidine resistance (a…

A: The inventors of fluctuation test were Delburk and Luria in 1943. This test aimed to determine…

Q: The carboxy terminus of the p53 protein acts as an allosteric regulator of sequence-specific DNA…

A: General structure of p53 protein

Q: on a gene in a microorganism that provides a characteristic you find interesting. However, I want it…

A: Microbes are one of the smartest living entities in this living world. As a part of evolution, they…

Q: What is the actual biological purpose of the CRISPR/Cas9 system?

A: All biological information of an organism is stored inside the biomolecule called DNA.  Gene are the…

Q: In threonine selected marker number of co-transformation rate is Met 2%-Gly 60%- Trp 10%. In…

A: Introduction: When transformation of two or more genes occur together simultaneously then this…

Q: What technique would you use for each senario(a and b) and why. FISH, immunofluorescence, and GFP…

A: The use of cytogenetic analysis as part of diagnostic procedures for various human conditions is…

Q: 1) In MALDI-TOF data, when performing peptide mass mapping search, a) The number of peptides…

A: Protein identification with the help of mass spectrometry (MS) is an major step in proteomic…

Q: Consider E, coli cells(i) with no vector,(ii) transformed with PBR322, or (iii) transformed with a…

A: pBR322 is a plasmid vector. It is double standard DNA molecule. It contains- Origin of replication…

Q: steps you would follow to generate a recombinant plasmid that can be used in downstream…

A: Plasmids are extra chromosomal material that exist independently of the main chromosome.  They may…

Q: Knock out mice that are mutant for a gene product X die. Variants of the X gene that were…

A: The physical crossing over during meiosis of parental heterozygous homologous chromosomes or genes…

Q: Assume that an organism has 400 V and 10 J light chain gene segments and 400V, 10 D, and 10 J heavy…

A: Introduction The rearrangement of V, D or J combinations results in the formation of the V region of…

Q: was reduced in the knock-out mutant cells. The N-terminal region of Protein X is 100% conserved…

A: As per our policy we are answering the first three sub parts ( a, b , c ). Of the Question For the…

Q: A 2.0kb bacterial plasmid ‘BS1030’ is digested with the restriction endonuclease Sau3A; the plasmid…

A: Plasmids are small, circular, double stranded, and extrachromosomal bacterial DNA. i.e. it is…

Q: 28. In a transformation experiment, DNA is collected from an E. coli donor strain of genotype cys(+)…

A: There are three modes of transfer of genetic information in bacteria. They are transduction,…

Q: Several DNA coding for different proteins, CRISPR, or siRNAs against different genes were expressed…

A: The given experimental setup involves the expression of various proteins and treatments using siRNAs…

Genomic studies

Genomic studies (genomics) is the study of the whole genome of an organism. Using bioinformatics and high-performance computing, genomic researchers examined massive amounts of deoxyribonucleic acid (DNA)-sequence data to discover variations or mutations that affect drug response, health, or disease. Genomics is a much younger discipline than genetics, having only emerged in the last few decades as a result of technological advancements in DNA sequencing and computational biology.