Among the following statements regarding the use of viral vectors, which is (are) true: (can be more then one answer) A.Viral vector transfection has the defect of rapidly killing cells, given the high toxic potential of viruses. B.Generally, the effectiveness of a viral infection transfection is much higher than an electroporation or cationic liposome transfection. C.Even before infecting a cell, a virus can begin to express the indicators, accelerating the process of expression of the indicator in the culture medium. D.Adeno-associated viruses are little used in biophotonics, as they require simultaneous adenovirus infection.
Among the following statements regarding the use of viral vectors, which is (are) true: (can be more then one answer)
A.Viral vector transfection has the defect of rapidly killing cells, given the high toxic potential of viruses.
B.Generally, the effectiveness of a viral infection transfection is much higher than an electroporation or cationic liposome transfection.
C.Even before infecting a cell, a virus can begin to express the indicators,
accelerating the process of expression of the indicator in the culture medium.
D.Adeno-associated viruses are little used in biophotonics, as they require simultaneous adenovirus infection.
Transfer of foreign nucleic acid insert into the eukaryotic cell is transfection. For many scientific research purposes, the desired nucleic acid sequence has been introduced into the eukaryotic host genome to develop the desired gene product by using the transfection method. Modern life-scientific research uses different transfection techniques and this search for better transfection tools/methods/ technique is in a continuous ever-refining way.
The use of viral vectors to transfect eukaryotic cells is now a good and reliable approach by researchers. Although each approach has its own utilities and limitations.
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