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Your friend hasn't been feeling well, and you think that she should get tested because it could be a virus or even multiple viruses! Nancy is conducting a qPCR experiment to detect how much of virus A, B, and/or C your friend is infected with. All three viruses contain RNA as their genetic material. Nancy takes a saliva sample and is excited to begin. However, it's her very first time conducting qPCR so a few mishaps occurred during this experiment. Unfortunately... • Nancy forgets to reverse transcribe the RNA of virus A, and • The cDNA of virus C contains a mutation where the probe is designed to bind, and • Nancy uses the same color fluorescent dye for viruses B and C Fortunately, all the probes consisted of a fluorescent and quenching dye. Which qPCR graph is most representative of Nancy's experiment after several cycles of qPCR? Explain why the answer you chose is correct. A. (₁ Fluorescence Fluorescence. B ↓ B # of Cycles A T T A # of Cycles B. D.. Fluorescence. Fluorescence # of Cycles B # of Cycles

Your friend hasn't been feeling well, and you think that she should get tested because it could be a virus or even multiple viruses! Nancy is conducting a qPCR experiment to detect how much of virus A, B, and/or C your friend is infected with. All three viruses contain RNA as their genetic material. Nancy takes a saliva sample and is excited to begin. However, it's her very first time conducting qPCR so a few mishaps occurred during this experiment. Unfortunately... • Nancy forgets to reverse transcribe the RNA of virus A, and • The cDNA of virus C contains a mutation where the probe is designed to bind, and • Nancy uses the same color fluorescent dye for viruses B and C Fortunately, all the probes consisted of a fluorescent and quenching dye. Which qPCR graph is most representative of Nancy's experiment after several cycles of qPCR? Explain why the answer you chose is correct. A. (₁ Fluorescence Fluorescence. B ↓ B # of Cycles A T T A # of Cycles B. D.. Fluorescence. Fluorescence # of Cycles B # of Cycles

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Your friend hasn't been feeling well, and you think that she should get tested because it could be a virus or even multiple viruses! Nancy is conducting a qPCR experiment to detect how much of virus A, B, and/or C your friend is infected with. All three viruses contain RNA as their genetic material. Nancy takes a saliva sample and is excited to begin. However, it's her very first time conducting qPCR so a few mishaps occurred during this experiment. Unfortunately... ● Nancy forgets to reverse transcribe the RNA of virus A, and • The cDNA of virus C contains a mutation where the probe is designed to bind, and • Nancy uses the same color fluorescent dye for viruses B and C Fortunately, all the probes consisted of a fluorescent and quenching dye. Which qPCR graph is most representative of Nancy's experiment after several cycles of qPCR? Explain why the answer you chose is correct. A. (₁ Fluorescence B B # of Cycles A A # of Cycles с B. D. Fluorescence B # of Cycles B # of Cycles с
Transcribed Image Text:Your friend hasn't been feeling well, and you think that she should get tested because it could be a virus or even multiple viruses! Nancy is conducting a qPCR experiment to detect how much of virus A, B, and/or C your friend is infected with. All three viruses contain RNA as their genetic material. Nancy takes a saliva sample and is excited to begin. However, it's her very first time conducting qPCR so a few mishaps occurred during this experiment. Unfortunately... ● Nancy forgets to reverse transcribe the RNA of virus A, and • The cDNA of virus C contains a mutation where the probe is designed to bind, and • Nancy uses the same color fluorescent dye for viruses B and C Fortunately, all the probes consisted of a fluorescent and quenching dye. Which qPCR graph is most representative of Nancy's experiment after several cycles of qPCR? Explain why the answer you chose is correct. A. (₁ Fluorescence B B # of Cycles A A # of Cycles с B. D. Fluorescence B # of Cycles B # of Cycles с
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