2). a). You mutagenize phage X and obtain three mutants that no longer cause plaques when incubated (individually) with E. coli strain K12. You cross these phage mutants together in a cis test and each cross produces low numbers of plaques in K12 E. coli. Are these mutants allelic? Why or why not? b). You perform a trans test between two of your mutant X phage (X¹ and X²). You observe no progeny phage when you plate the coinfection supernatant onto a lawn of K12 E. coli. Repeating complementation tests between X¹ or X² and the parental X phage (i.e. X¹ x X or X² x X) produce plaques on K12 E. coli. Are these mutants allelic? Why or why not?
2). a). You mutagenize phage X and obtain three mutants that no longer cause plaques when incubated (individually) with E. coli strain K12. You cross these phage mutants together in a cis test and each cross produces low numbers of plaques in K12 E. coli. Are these mutants allelic? Why or why not? b). You perform a trans test between two of your mutant X phage (X¹ and X²). You observe no progeny phage when you plate the coinfection supernatant onto a lawn of K12 E. coli. Repeating complementation tests between X¹ or X² and the parental X phage (i.e. X¹ x X or X² x X) produce plaques on K12 E. coli. Are these mutants allelic? Why or why not?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:2). a). You mutagenize phage X and obtain three mutants that no longer cause plaques when
incubated (individually) with E. coli strain K12. You cross these phage mutants together in a cis
test and each cross produces low numbers of plaques in K12 E. coli. Are these mutants allelic?
Why or why not?
b). You perform a trans test between two of your mutant X phage (X¹ and X²). You observe no
progeny phage when you plate the coinfection supernatant onto a lawn of K12 E. coli.
Repeating complementation tests between X¹ or X² and the parental X phage (i.e. X¹ x X or X² x
X) produce plaques on K12 E. coli. Are these mutants allelic? Why or why not?
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