Sam is working in a genetics lab this summer to gain more hands-on experience before applying to graduate school. The professor with whom he is working is particularly interested in studying a new strain of E. coli and assigns Sam an interrupted mating experiment to perform. Sam is attempting to determine the order of 7 different genes on the bacterial chromosome: Ger, Fra, Lod, Moc, Bla, Jat, and Arp. Sam used four different Hfr strains (Hfr1, Hfr2, Hfr3, Hfr4) to help him determine the gene order. He observed that each Hfr strains transferred genes at the times shown in the table below. However, Sam is a little forgetful and neglected to record the times at which several of the genes were transferred. Afraid that the professor will become angry with him, Sam comes to you for assistance. Using the data from the experiment; fill in the missing data in the table attached and draw a single circular map of the seven genes as they would appear on the E. coli chromosome. Be sure to include all of the following: Genes appear in the correct order Distances between adjacent genes (in minutes) (Correct distances between genes) Distance between genes and origin of transfer for each Hfr (in minutes) (Correct distance between integrated Hfr and first gene for each Hfr) Location of each of the 4 integrated F factors (origin and direction of transfer for each Hfr) (Correct position and orientation Hfr)
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Sam is working in a genetics lab this summer to gain more hands-on experience before applying to graduate school. The professor with whom he is working is particularly interested in studying a new strain of E. coli and assigns Sam an interrupted mating experiment to perform. Sam is attempting to determine the order of 7 different genes on the bacterial chromosome: Ger, Fra, Lod, Moc, Bla, Jat, and Arp. Sam used four different Hfr strains (Hfr1, Hfr2, Hfr3, Hfr4) to help him determine the gene order. He observed that each Hfr strains transferred genes at the times shown in the table below. However, Sam is a little forgetful and neglected to record the times at which several of the genes were transferred. Afraid that the professor will become angry with him, Sam comes to you for assistance.
Using the data from the experiment; fill in the missing data in the table attached and draw a single circular map of the seven genes as they would appear on the E. coli chromosome. Be sure to include all of the following:
- Genes appear in the correct order
- Distances between adjacent genes (in minutes) (Correct distances between genes)
- Distance between genes and origin of transfer for each Hfr (in minutes) (Correct distance between integrated Hfr and first gene for each Hfr)
- Location of each of the 4 integrated F factors (origin and direction of transfer for each Hfr) (Correct position and orientation Hfr)
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Would you be able to draw a circular map of the seven genes? I have trouble with correct position of the genes
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