You perform an Ames test to determine which compound X causes mutations or not. You grow an auxotrophic strain of bacteria on three plates; one with no compound X, one with just compound X and one with compound X mixed with liver enzymes. You find many more colonies on the plates with compound X and with compound X plus liver enzymes compared to the control plate. Which of the following describes the best interpretation of the results? O Compound X causes mutations on it's own but is safe after exposure to liver enzymes. O Compound X is not mutagenic. O Compound X is mutagenic regardless of whether it has been processed by liver enzymes. O Compound X is mutagenic only after being treated with liver enzymes.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Technique is developed by bruce ames.
Ames test is generally used to detect the mutation in the test organism ( bacteria) by growing those species in the plate in the presence of certain chemicals. If in case the particular chemical that is used in the plate that has a ability to induce a mutation in the bacteria, then the bacteria will grow more in the plate compared to the control plate which do not contain the chemical.
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