SOD1 vs RM95 – 20% recombinant TGLA57 vs UMPS – 21% recombinant UMPS vs CRYG8- 17% Recombinant RM95 vs UMPS 50% - recombinant TGLA vs CRYG8 – 38% recombinant SOD1 vs TGLA57 – 49% recombinant RM95 vs TGLA 57 – 29% recombinant SOD1 vs UMPS – 50% recombinant what is the order of the genes on the chromosome and what is there distance in cM

Q: In several experiments, you find these orders of genes getting transferred in from the chromosome…

A: F plasmid is a type of plasmid which allows the transfer of genes from one bacteria to the other…

Q: Describe the banding pattern seen in the gel. What does that tell you about the role of annealing…

A: Gel electrophoresis is a laboratory technique for separating charged molecules such as DNA, RNA, and…

Q: Recombination signal sequences are conserved heptamer and nonamer sequences that flank the V, J, and…

A: Introduction: RAG 1 & 2 are the recombination activating genes that encode a portion of a…

Q: Indicate 2 differences between SSLP (simple sequence length polymorphism) and SNP (single nucleotide…

A: SSLPs are called Simple Sequence Length Polymorphism. These are arrays of repeat sequences that…

Q: hich repair mechanisms is most require to fix thymine dimers? BER NER mismatch repair…

A: When many proteins and RNAs are damaged, they will be degraded and sugars, are metabolized in order…

Q: DNA polymerase I DNA polymerase II DNA ligase Primase RNA primer 5' Lagging strand 3' 3' Okazaki…

A: The process of copying of double stranded DNA by the application of several Enzymes and proteins…

Q: In the Western blot shown here, proteins were isolated from redblood cells and muscle cells from two…

A: Thalassemia is a blood disorder passed down through families (inherited) in which the body makes an…

Q: In Figure 17-26, the bottom panel shows that genes B andC are oriented in a different direction…

A: Inversion has many effects on genes either it has no effect or disrupts the gene function. This…

Q: Why can’t a linear duplex DNA, such as that of bacteriophage T7, be fully replicated by just E.…

A: There are two replication systems present in organisms, namely linear DNA replication system and…

Q: In the gel electrophoresis: the mutant would just run the same as the open circle DNA if it simply…

A: In gel electrophoresis, the behavior of mutant DNA in comparison to open circle DNA and the presence…

Q: During conjugation,. HFR; F+ function as DNA donors and, function as DNA recipients. F+; F- F-; F+…

A: Conjugation is a type of horizontal gene transfer seen in prokaryotes.

Q: "The antibody diversity created by the combinatorial joining of V, D, and J segments by V(D)J…

A: Early phases of T and B lymphocyte development are characterised by V(D)J recombination, which takes…

Q: When a genome is edited using the CRISPR/Cas9 system, a knockout allele can be produced if DNA is…

A: CRISPR: CRISPR  is a newly emerging tool that is used by scientists to alter the genome structure by…

Q: It was shown that the pan marker entered last in interrupted conjugation experiments. By spreading…

A: Minimal media is the media which required minimum component for the growth.  Here MM+ glucose = 280…

Q: pXO1 and pXO2 are large plasmids – large pieces of DNA that are not the chromosome that can…

A: Plasmids are the extra chromosomal material which are present independently of  the main…

Q: Bacteriophage λ, after infecting a cell, can integrate into the chromosome of the cell if the…

A: Temperate phages are bacterial viruses that as a part of their life cycle reside within the…

Q: What is the difference between an Hfr chromosome using normal excision and aberrant excision?

A: High frequency recombination (Hfr) cells are the bacteria having a conjugative plasmid (Fertility…

Q: Extreme UV exposure leads to the SOS response in bacteria. By what mechanism does the SOS response…

A: The SOS response is a DNA damage response mechanism in bacteria that is activated when the cell is…

Q: Kan selectable marker plant selectable marker constitutive promoter T-region conjugative transfer…

A: Agrobacterium tumefaciens is a soil bacterium that naturally infects plants and produces tumors by…

Q: Two pathways, homologous recombination and nonhomologous end joining (NHEJ), can repair…

A: Non-homologous end joining (NHEJ) is one important pathway in eukaryotic cells responsible for the…

Q: How many nucleotides are in the consensus coding sequence (CDS) of the KMT2D transcript?

A: A consensus coding sequence (CDS) is a method used in next-generation sequencing (NGS) to identify…

Q: DNA obtained from the indicated donor type recombinants are scored. The number of wild-types for…

A: Recipient lacking in b and c genes (a+ b-c-) has the largest number of transformed colonies(462

Q: A recombinant protein corresponding to 52 repeats of the peptide sequence YSPTSPS was covalently…

A: The peptide sequence YSPTSPS is a known phosphorylation site that corresponds to the heptad repeat…

Q: B: Lambda DNA/Hindlll Marker, 2 GeneRuler" 1 kb DNA Ladder O'GeneRuler" 1 kb DNA Ladder,…

A:

Q: E. coli strains diploid for the lac region were constructed by introducing a plasmid carrying the…

A: ANSWER;- I- mutation- repressor is unable to bind to the operator region. If there is no other…

Q: An Hfr strain that is hisE + and pheA + was conjugated to a strain that is hisE − and pheA −. The…

A: Conjugation experiments are done to map different genes in bacteria. The mapped distances determined…

Q: An Hfr strain that is lacZ and galE" was mated to a strain that is lacZ and galE. The mating was…

A: Introduction: The transfer of the genetic material from one bacterium to another via the conjugation…

Q: F ′strains in E. coli are derived from Hfr strains. In some cases, these F ′strains show a high rate…

A: Conjugation is transfer of genes in bacteria.cell to cell contact by F-pilus or sex pilus allows the…

Q: Why and how do the polymerase go backward from state 2 (post-translocation state) to state…

A: During replication and transcription, the polymerase enzyme moves along the DNA template to…

Q: Based on the following wild type DNA sequence, indicate if each of the mutations should be…

A: A mutation is a change in a DNA sequence. Mutations can result from DNA copying mistakes made during…

Q: DNA saquance of interest DNA of interest Open vector Vector containing DNA of interest -Vector DNA…

A: When a genetically changed vector is injected and integrated into the genome of an organism (host),…

Q: An Hfr strain that is hisE+ and pheA+ was mixed with a strain thatis hisE− and pheA−. The…

A: Transfer of genes in prokaryotes occurs through horizontal transfer mechanisms such as transduction,…

Q: For the following sequence please design an 18 base pair forward primer.  ATG TCA AAA GCT GTC GGT…

A: Introduction :  Primers are short oligonucleotides that are chemically synthesised and complementary…

Q: Different Hfr strains have the F factor DNA integrated into their chromosome at different locations…

A: The integration of the F factor DNA into the chromosome of Hfr (high-frequency recombination)…

Q: 12) Draw a yeast knockout cassette. Label all required sequence features.  a) Draw the target…

A: Hi! As you have posted multiple questions and asked to provide with two knockout diagrams, we are…

Q: SOD1 vs RM95 – 20% recombinant TGLA57 vs UMPS – 21% recombinant UMPS vs CRYG8- 17% Recombinant RM95…

A: Genetic linkage is explained as the closeness of genes on a chromosome (closely packed DNA molecule)…

Q: DNA obtained from the indicated donor strains is used to TRANSFORM the indicated recipient strains.…

A: The correct answer is option C. Arginine The arginine locus is closest to serine.

Q: Primer pairs of homo sapiens isolate breast cancer brca1 gene. Select the best primer pair then give…

A: Primers are used in PCR techniques which enable us to generate several copies of a desired DNA…

Q: Recombinant protein production by a genetically modified Escherichia coli strain is proportional to…

A: In this scenario, we explore aspects of recombinant protein production using a genetically modified…

Q: An Hfr strain that is hisE+ and pheA+ was mixed with a strain thatis hisE− and pheA−. The…

A: Transfer of genes in prokaryotes occurs through horizontal transfer mechanisms such as transduction,…

Mitochondrial Mutations

A mitochondrion is a cellular organelle that serves as the site for cellular respiration and energy production (ATP or Adenosine Triphosphate). It is a double-membrane-bound organelle and is only present in eukaryotic cells.