As you are performing this protocol, you realize that the ultraviolet (UV) light does not work as expected, because of a fused bulb. You decide to look at plate #4 (NA with AMP; pGFP added to cells) under visible light to decide whether transformation was successful or not. Will viewing this plate under visible light tell you whether you were successful in transforming cells with pGFP DNA?
Nucleotides
It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic Acids
Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.
Introduction
A gene is a DNA segment that encodes for protein or RNA (ribonucleic acid). It is the basic functional and physical unit of heredity. The gene size varies from a few hundred base pairs of DNA to greater than 2 million base pairs in humans. The human genome contains around 25000 genes.
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