Testing complementation by using a heterokaryon arg-1 cells, defective for one specific enzyme in arginine synthetic pathway arg-2 cells, defective for a different enzyme in arginine synthetic pathway Fusion Heterokaryon grows without arginine FIGURE 6-13 A heterokaryon of Neurospora and similar fungi mimics a diploid state. When vegetative cells fuse, haploid nuclei share the same cytoplasm in a heterokaryon. In this example, haploid nuclei with mutations in different genes in the arginine synthetic pathway complement to produce a Neurospora that no longer requires arginine.
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
In Figure 6-13, explain at the protein level why this heterokaryon can grow on minimal medium
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