Suppose E. coli is growing in a growth medium containing lactose as the sole source of carbon. The genotype is i- z+ y+. Glucose is then added. Which one of the following will happen? (Only one answer is correct)
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- In your laboratory, you have an F− strain of E. coli that is resistantto streptomycin and is unable to metabolize lactose, but it can metabolizeglucose. Therefore, this strain can grow on a medium thatcontains glucose and streptomycin, but it cannot grow on a mediumcontaining lactose. A researcher has sent you two E. colistrains in two separate tubes. One strain, let’s call it strain A, hasan F′ factor (an F prime factor) that carries the genes that are requiredfor lactose metabolism. On its chromosome, it also has thegenes that are required for glucose metabolism. However, it is sensitiveto streptomycin. This strain can grow on a medium containinglactose or glucose, but it cannot grow if streptomycin is addedto the medium. The second strain, let’s call it strain B, is an F−strain. On its chromosome, it has the genes that are required forlactose and glucose metabolism. Strain B is also sensitive to streptomycin.Unfortunately, when strains A and B were sent to you, thelabels had fallen…A phenylgalactoside-type compound, which yields galactose when enzymatically cleaved, was added as the sole carbon source to petri dishes containing E. coli culture medium. E. coli can utilize galactose for growth. Each of the following bacterial strains was plated on this medium and scored for growth after 1-2 days. Growth Genotype lacz+ lacI+ lacz+ lacIs lacz+ lacl- + oc lacZ+ lacI+ + a) What do you conclude about this compound with respect to its ability to be cleaved by ß- galactosidase? Why? b) What do you conclude about this compound with respect to its ability to inactivate the Lac repressor? Why? TYou have several different media onto which you inoculated eight strains of yeast (A-H). The media include a rich medium, an unsupplemented minimal medium, and minimal media each supplemented with one vitamin. Of the yeast strains, one is a prototroph and seven are auxotrophs for a vitamin. After overnight incubation, the following results were observed (tan patches represent growth): D plate 1 (A) B DE F GH plate 5 plate 4 plate 6 Which plate contains an unsupplemented minimal medium? [Select] Which plate contains a rich medium? [Select] plate 2 Which strain is a prototroph? [Select] Strain E is an auxotroph for niacin. Which plate reveals this specific auxotrophy? [ Select] plate 3 plate 7 One strain is an auxotroph for both choline and pantothenic acid. Which one is this most likely to be? [Select]
- In the table, you have to identify the character for Genus Identification of the Cyanobacterium E (see image). Thank you. You did not answer what was asked in the table above, you only defined or gave the meaning of the morpho-cytological characteristics which was not asked.Explain why the growth of both the strains A and C in medium III is not similar to that of strain B. Use the appropriate vocabulary and provide one putative explanation for strain A.Each Escherichia coli cell weighs 5ng. How many cells are present in a colony that weighs 1mg? Write only the number with any needed commas or decimals. Do not include units.
- The correct answer for this homework problem is D because the sythase is moving that direction so it can't be the starting point, right?Report the slope and the y-intercept below. Slope () = = y-intercept () = Can this standard curve be used for E.coli cells (why/why not?)Fill in the following table with the size of the listed cell types. Search in your text and perform online searches to gather the information. You may not find these in the same units. Report wherever units you find but be sure to include a unit (diameter, cc,, etc). You must include the source of your information. (for example: www.ncib.nlm.nih.gov) Cell Type E. coli cell Corbeely Com Bacillus subtilis cell Human skin cell Human brain cell Euglena Palisade Cell Measuring and Estimating Cell Size Mitochondria Influenza particle COVID particle Size (include units) DER Your Source
- In an Hfr × F− conjugation, genes can be mapped roughlyby the time at which different alleles from the Hfr donorsfirst appear in F− exconjugants, and more precisely bycounting the exconjugants of each _________ classTRY TO KEEP IN SHORT AND USE OWN WORD FOR THIS QUESTION You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?The wild type bacterium is lac+ amps, leu+, ade+ Mutant I is: lac+ amp r, leu-, ade+ Mutant 2 is: lac+, amp s, leu+, ade- Mutant 3 is: lac-, amp r. leu-, ade- The circles below represent six different types of growth medium for these cells. The text below each circle indicates the composition of the medium in that plate. Please divide each plate into quadrants, one for the wild type and one each for the three mutants. Then, draw in the appearance of the plates after we place cells of these genotypes on them and allow growth to occur (if it can). rich, glucose rich, lactose OO minimal, leucine, adenine minimal, glucose, adenine rich, lactose, amp O minimal. glucose, leucine