methionine, just in case your yeast happens upon circumstances where it can’t eat methionine. It seems wasteful to produce these proteins all the time though. Why? Question 2) In order to not waste a bunch of energy and materials making proteins to make methionine when the yeast doesn’t need to make methionine. You organize the DNA so that the genes are controlled by a promoter. Which promoter makes sense and why? i) lac promoter, turns on when lactose is present. ii) Pmet3 promoter, turns off when methionine is present. iii) pbr promoter, turns on when lead is present.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Question 1) Imagine you’re making a yeast from scratch, and you’re worried your yeast may sometimes not have access to the amino acid methionine. You decide to put some genes in that code for proteins that allow it to make methionine, just in case your yeast happens upon circumstances where it can’t eat methionine. It seems wasteful to produce these proteins all the time though. Why?
Question 2) In order to not waste a bunch of energy and materials making proteins to make methionine when the yeast doesn’t need to make methionine. You organize the DNA so that the genes are controlled by a promoter. Which promoter makes sense and why?
i) lac promoter, turns on when lactose is present.
ii) Pmet3 promoter, turns off when methionine is present.
iii) pbr promoter, turns on when lead is present.
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