How much 4x loading dye should be added to 11 uL of DNA? Report you answer in units of uL, with one digit after the decimal point
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How much 4x loading dye should be added to 11 uL of DNA? Report you answer in units of uL, with one digit after the decimal point.
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- Make 2 mL of 50 fold dilution of DNA solution and sodium phosphate buffer. DNA: 400 uL Sodium phosphate buffer, pH 6.9, 10mL - Calclate amount of DNA & buffer need to make dilutionFor a DNA sample, the OD value at 260nm = 0.125, and the OD value at 280nm 0.065. Purity of DNA - OD 260/280 %3D Concentration of DNA = Abs x 50 µg/ml x dilution factor (100) 0.125 x 50 µg/ml x 100 = Edit View Insert Format Tools Table 12pt v Paragraph v BIUA 2 T'v1). What is the concentration of a solution of DNA with an OD260 of 0.3? ----I got 15 micrograms/mL (50 x 0.3) 2). How many microliters of this solution of DNA would you need if you wanted 1.5 micrograms?
- A sample of DNA with the sequence 5'- CTC GAG CGA AGC TCA ACC-3' he was obtained as a dry solid. The sample was dissolved in 1000 µl of deionized water ane mixed well. Ten microliters of the dissolved sample was then transferred to a new sampie tube and mixed with 990 ul of water, The dilute sample had A260 = 0.156. What was the concentration of the original sample (the solid dissolved in 1000 ul of deionized water)t (5 a 1 dao) - Google Chrome edu.om/mod/quiz/attempt.php?attempt=2334320&cmid=1127808 SQU E-LEARNING SYSTEM (ACAI الوقت المتبقي 0.175mm .e O In the DNA extraction experiment, the separation of DNA from its packaging protein can be achieved by the addition of Detergent aO 1.00 Salt b O هذا السؤال Ethyl alcohol cO Papain powder .d O Cold water Le O In paramecium, osmoregulation occurs through 3 Activate Windowssk Macronucleus Goaotings to acttivate do nere to search 6. 70°F Mostly sunny 9:05 ENG 12/18 f2 O f3 O f4 O f6 O f8 end f7 0 F10 insert f9 D F12 T num IA break @ %24 & 8 2 3 r 4 5 6 7 8 A 9 9 R Y ! U S F G J K +|| 回 Q 以 4What is the structure of the gel stain being used? There are several other dyes that can bind DNA and used for gel imaging. Find an example of such a dye, and draw its structure.
- Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes. Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…The concentration of a DNA sample is 40 nano grams per milliliter how many nanoliters will be needed to obtain 0.5 nano grams of DNAUsing a 1 cm cuvette, the absorbance at 260nm of your double-stranded DNA sample is 0.15. What is the concentration of your sample?
- Both protein and DNA are run together in an isoelectric focusing (IEF) electrophoresis using the immobilised pH gradient (IPG) strip with pH range of 4-7. After the electrophoresis and staining, only ONE band is observed on the middle of the IPG strip. The band is a protein band. Briefly explain why only the protein band and NOT the DNA band appear on the IPG strip.Aa Po AaBbCcDc AaBbCcDdEe AaBbCcDdE AaBbCcDdE AaBbCcDdE BIUVab x, x A ev A 三==|這、|の Caption Emphasis Heading 1 Heading 3 Heading 4 Style Pane (a) Complete the table, using a letter D, R or B, to show whether each statement applies to: Fo DNA only (D) TA2G – Completed forms must be available for Open Awards external moderation purposes. Page 6 of 15 > T | RNA only (R) Both DNA and RNA (B) Statement D or R or B Contains thymine Contains ribose Consists of two chains connected to each other with hydrogen bonds Has a sugar-phosphate backbone Has four different nitrogenous bases Contains a pentose sugar Is found in the nucleus and cytoplasm English (United Kingdom) Focus 目民 2490 words +1One OD unit of A260 is 50 ug/ml dsDNA. You have 2.75 ml genomic DNA (stock). You took 50 ul of the DNA sample and mixed it with 950 ul water and measured the A260 absorption. The diluted sample absorbed 0.14 units of A260. The DNA concentration of the stock is ug/ml, considering the dilution factor of (Note: ug microgram, ul- microliter). 70/10 140/20 280/50 O 560/100
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