After properly troubleshooting, you finally are able to visualize the gel. You obtained the image below. The ladder used is “Lambda Hind III”. 1. Looking at the gel results, what can you say about the quality of DNA extraction for protocols 1, 2 & 3? 2. What would you take into consideration when selecting a molecular marker or leader to include in your gel run?
After properly troubleshooting, you finally are able to visualize the gel. You obtained the image below. The ladder used is “Lambda Hind III”.
1. Looking at the gel results, what can you say about the quality of DNA extraction for protocols 1, 2 & 3?
2. What would you take into consideration when selecting a molecular marker or leader to include in your gel run?
DNA extraction is a protocol of isolating genomic DNA from any cell type. Generally we use the principle of Phenol-chlorofoam extraction in order to isolate genomic DNA after lying the cell of interest.
The used Ladder Lambda Hind III is a high molecular weight ladder with highest band around 23kb followed by 9.5kb, 6,5kb and 4.5 kb bands. The ladder or gel marker is used to get reference of the DNA size that is run on the gel. WE select appropriate markers depending upon the estimated size of DNA/RNA that we wish to visualize on the gel.
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