An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel, while 10 ul of the DNA samples plus an appropriate amount of 6x loading buffer were added into the gel. Shown below is the AGE profile and the needed marker details where M is the MW marker while numbers 1-4 indicate the lanes with the DNA samples: M 2 3 4 ng/0.5 µg bp 5000 20 4000 40 3000 20 2000 20 1000 60 500 100 Compare the bands in the Lanes 1,2,3,4 with the nearest DNA standard by determining the DNA standard which is nearest in size to the samples in Lanes 1,2,3,4 DNA standard (choices: 100, 500, 1000, 2000, 3000, 4000, or 5000 bp) DNA sample Lane 1 bottom band Lane 1 top band Lane 2 band Lane 3 band Lane 4 band 20 20
An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel, while 10 ul of the DNA samples plus an appropriate amount of 6x loading buffer were added into the gel. Shown below is the AGE profile and the needed marker details where M is the MW marker while numbers 1-4 indicate the lanes with the DNA samples: M 2 3 4 ng/0.5 µg bp 5000 20 4000 40 3000 20 2000 20 1000 60 500 100 Compare the bands in the Lanes 1,2,3,4 with the nearest DNA standard by determining the DNA standard which is nearest in size to the samples in Lanes 1,2,3,4 DNA standard (choices: 100, 500, 1000, 2000, 3000, 4000, or 5000 bp) DNA sample Lane 1 bottom band Lane 1 top band Lane 2 band Lane 3 band Lane 4 band 20 20
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul)
was loaded into the gel, while 10 ul of the DNA samples plus an appropriate
amount of 6x loading buffer were added into the gel.
Shown below is the AGE profile and the needed marker details where M is the
MW marker while numbers 1-4 indicate the lanes with the DNA samples:
M
2
3
4
ng/0.5 µg
bp
5000
20
4000
40
3000
20
2000
20
1000
60
500
100
Compare the bands in the Lanes 1,2,3,4 with the nearest DNA standard by determining the DNA
standard which is nearest in size to the samples in Lanes 1,2,3,4
DNA standard (choices: 100, 500, 1000,
2000, 3000, 4000, or 5000 bp)
DNA sample
Lane 1 bottom band
Lane 1 top band
Lane 2 band
Lane 3 band
Lane 4 band
20
20
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