You start running your gel and after a few minutes notice that the colored bands from the loading dye are moving in the wrong direction. This is happening because you accidentally: O Forgot to add loading dye to your DNA samples O Forgot to add ethidium bromide to your DNA samples O Attached the negative electrode to the gel box on the end where samples were loaded Attached the positive electrode to the gel box on the end where samples were loaded

Q: An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel,…

A: if 3ul of DNA ladder is added on the gel AGE which has a concentration of 100ng/ ul. Then it means :…

Q: When you use the centrifuge the second time with the alcohol, where does the DNA go?

A: The type of nucleic acid known as DNA (Deoxy Ribo Nucleic Acid) controls the growth, metabolism, and…

Q: Lvris and Irelation of DNA from Ranana)

A: To study the genetic build up of an organism, it is important to know the traits it possesses. Based…

Q: DNA Fingerprints Cat Kittens IL || ILL || I I

A: DNA fingerprinting is a technique used to compare sequences in genomes using satellite DNAs like…

Q: A DNA fragment with 450 bp will be closer to the top (negative pole) of an electrophoresis gel than…

A: Friedrich Meischer discovered nucleic acids from the nuclei of pus cells and coined the term…

Q: Which of the following correctly describes a possible scenario during a run of gel electrophoresis…

A: Agarose Gel electrophoresis(AGE) is a method used in molecular biology to separate mixed RNA, DNA,…

Q: researcher runs an agarose gel IIl ladder one läñé ând 100 ng of a 500 bp DNA fragment in another…

A: Agarose gel electrophoresis or AGE is a form of gel electrophoresis that is used for discriminate…

Q: A plant isolate was subjected to qualitative tests to determine the presence of macromolecules.…

A: Biological macromolecules are constituted of proteins , nucleic acids (DNA or RNA), carbohydrates…

Q: Spin 1.5 ml of cells in a microcentrifuge at maximum speed (12,000 rpm) for pellet. Remove the…

A: To isolate plasmid DNA,  cells are cracked open and a mini preparation should be performed, trying…

Q: You now redo the experiment but instead of an SDS gel you run a native gel followed by completing…

A: Western blotting is the technique of identification of a specific protein in a given sample.

Q: 6. Identify the DNA sequence below. Write the DNA sequence in 5' to 3' orientation.

A: The DNA (deoxyribonucleic acid) sequence is the order in which the nucleotide bases are arranged.…

Q: An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel,…

A: Lane 1 bottom band has below 500 basepairs. Lane 1 top band has 5000bp. Lane 2 band 1000bp.

Q: a.What is the importance of centrifugation in step 3?  b.

A: Introduction: For the development of diagnostics and medications as well as for research into the…

Q: The concentration of a DNA sample is 40 nano grams per milliliter how many nanoliters will be needed…

A:

Q: DNA is charged and _fragments travel faster than positively, shorter, longer negatively, shorter,…

A: DNA is a polymer that is composed of two polynucleotide chains that coil around each other but…

Q: Which of the following gels is the preferred choice for separation of DNA fragments?  Agarose…

A: There are several techniques which are used in recombinant DNA technology. In a recombinant DNA…

Q: In this gel, which letter (A, B, or C) indicates the largest piece of DNA in lane one? If the…

A: Gel Electrophoresis is a separation technique for the DNA fragments based upon their size. When the…

Q: If the primers you purchased possessed the following information. 5'-GGA AAC AGC TAT GAC CAT G-3'…

A: Introduction :- All living things require primers, which are brief, single-stranded nucleic acids,…

Q: True or false: During DNA isolation, ice cold 95% ethanol is used to digest the DNA into fragments…

A: During DNA isolation 95% ethanol is used for DNA precipitation not for DNA digestion and while we…

Q: Aside from the sample and assuming proper AGE protocol, which of the following is most needed to…

A: Agarose gel  electrophoresis is an important technique used in molecular biology research. In case…

Q: What is the size of the DNA sample based on the gel profile below? Make sure to indicate the units.…

A: Electrophoresis is the technique to separate biomolecules depending on their…

Q: Loading dye stains the DNA molecules and thus help to visualize it under UV light apparatus. O True…

A: DNA Loading dye is used to monitor the migration of DNA into the gel during electrophoresis.…

Q: 4. Determine the 260/280 ratios for each of the four samples. Record these values in Table 2.

A: Absorbance is the most common method to measure the purity of DNA yeild. This method is simple,…

Q: What will happen to the concentration and the A260/280 ratio of the DNA sample in the following…

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

Q: How much 6x loading dye should be added to 49 uL of DNA?  Report you answer in units of uL, with one…

A: The equation of dilution: M1xV1 = M2xV2 Where, M1 is the molar concentration of the stock solution.…

Q: what is the incident beam  it is the beam of light that exits the sample it is the beam of light is…

A: Spectrophotometry is a technique used to measure light absorption or the amount of chemicals in a…

Q: 2. Discuss the mechanism of ethidium bromide (EtBr) and why is it used in staining DNA during…

A: "Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: This tool is related to doing a lab with DNA. It is used for checking DNA banding o laddering. Gel…

A: The theory behind gel electrophoresis is based on the principle that charged particles, such as DNA…

Q: Complete this Master Mix table for 8 DNA samples, a positive control, negative control, and an extra…

A: Volume per tube (uL) number of reactions volume in master mix (uL) PCR buffer 1.2 11 13.2 dNTP…

Q: Evaluate the gel provided below. Use the notes to help you read the gel: DNA is loaded in the wells…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Why does DNA appear when you add cold alcohol to the strawberry DNA solution?

A:

Q: Samples came to the laboratory for analysis. You are asked to identify the differences between these…

A: Spectrophotometer is used to determine the concentration of any unknown sample. DNA is a…

Q: 1 2 3 4 M 1000 b 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp 200 bp

A: The diagram is showing the separation of DNA fragments using gel electrophoresis.  In-gel…

Q: Which of the following conditions will affect the specificity of primer annealing? You may choose…

A: PCR stands for polymerase chain reaction, a technique used to make millions to billions of copies of…

Q: The sample furthest to the left contains the DNA size standards. What is the purpose of this sample?…

A: Gel electrophoresis is an analytical technique in which DNA fragments in a sample are separated…

Q: If you were to re-do this lab again with the same basic constraints (one DNA source, multiple…

A: DNA fragments are run on a agarose gel.

Q: How much 4x loading dye should be added to 11 uL of DNA?  Report you answer in units of uL, with one…

A: Recall the equation of dilution: C1 x V1 = C2 x V2 Where, C1 is the concentration of the stock…

Q: How would I make a calibration plot with gel electrophoresis? I LABLED THE SIDE WITH THE DNA…

A: In gel electrophoresis by applying an electric field to a gel matrix, DNA fragments migrate through…

Q: In which reagent is extracted DNA suspended to put it in solution? O Sodium dodecyl sulfate (SDS)…

A: Deoxyribonucleic acid is a polymer made up of two polynucleotide chains that coil around one another…

Q: The following gel shows the results of a crime scene investigation. Lane 1 shows a DNA sample that…

A: Gel electrophoresis:- it is a technique used for the separation of Nucleic acids i.e. DNA and RNA as…

Q: In a conventional PCR reaction cycle, which of the following step is NOT needed for a successful PCR…

A: Polymerase chain reaction (PCR) is a scientific method that exponentially multiplies a single copy…

Q: d)Halfway through the electrophoresis of your gel you realized that rather than using a buffer to…

A: D) As we know, the distilled water is neutral i.e. it doesn't contain any ions. Thus, when halfway…

Q: In lane 1, a size standard was loaded, which contained a mix a DNA fragments known to be 1000 bp,…

A: Gel electrophoresis is a gel technique that uses an applied electrical field to separate nucleic…

Q: step 4, isopropyl alcohol is used to precipitate out the DNA because it is not soluble. What does…

A: Introduction: Pure DNA is needed for DNA analysis. In cells, DNA can be found. However, cells also…

Q: Identify the component of the solution which is the extracted DNA

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Crime Scene Investigation

A crime scene refers to a place where a crime has occurred. The purpose of crime scene investigation (CSI) is to analyze the complete scene and catch the culprit based on the pieces of evidence collected from the place of crime. The people involved in this process are referred to as crime scene investigators.