You start running your gel and after a few minutes notice that the colored bands from the loading dye are moving in the wrong direction. This is happening because you accidentally: O Forgot to add loading dye to your DNA samples O Forgot to add ethidium bromide to your DNA samples O Attached the negative electrode to the gel box on the end where samples were loaded Attached the positive electrode to the gel box on the end where samples were loaded

Q: In this activity you will extract DNA from living material. Before you begin, here are some helpful…

A: DNA (deoxyribonucleic acid) extraction is the process in which DNA of the given living organism or…

Q: An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel,…

A: if 3ul of DNA ladder is added on the gel AGE which has a concentration of 100ng/ ul. Then it means :…

Q: Use the figure of the gel below to answer the following questions. Controls 1 2 3 Experimentals 4 5…

A: Gel electrophoresis is a technique used to separate DNA fragments,( or may be RNA and protein) based…

Q: In the next gel, in lane I is the marker. Estimate the sizes of DNA fragments (in base pairs, bp)

A: Electrophoresis is the migration of charged particles under the influence of electric current.…

Q: When you use the centrifuge the second time with the alcohol, where does the DNA go?

A: The type of nucleic acid known as DNA (Deoxy Ribo Nucleic Acid) controls the growth, metabolism, and…

Q: You have 10 ul of DNA you want to cut with HindIII, and then run a gel, in order to cut out certain…

A: Restriction Digestion is a process by which DNA molecules are cut into small pieces with the aid of…

Q: Lvris and Irelation of DNA from Ranana)

A: To study the genetic build up of an organism, it is important to know the traits it possesses. Based…

Q: A DNA fragment with 450 bp will be closer to the top (negative pole) of an electrophoresis gel than…

A: Friedrich Meischer discovered nucleic acids from the nuclei of pus cells and coined the term…

Q: Which of the following correctly describes a possible scenario during a run of gel electrophoresis…

A: Agarose Gel electrophoresis(AGE) is a method used in molecular biology to separate mixed RNA, DNA,…

Q: When gel electrophoresis is undertaken, one well always contains DNA-marker molecules. The purpose…

A: The objective of the question is to understand the purpose of DNA-marker molecules in a gel…

Q: researcher runs an agarose gel IIl ladder one läñé ând 100 ng of a 500 bp DNA fragment in another…

A: Agarose gel electrophoresis or AGE is a form of gel electrophoresis that is used for discriminate…

Q: A plant isolate was subjected to qualitative tests to determine the presence of macromolecules.…

A: Biological macromolecules are constituted of proteins , nucleic acids (DNA or RNA), carbohydrates…

Q: You now redo the experiment but instead of an SDS gel you run a native gel followed by completing…

A: Western blotting is the technique of identification of a specific protein in a given sample.

Q: do you need to put the reagents in the reaction tubes into the tubes in a specific order for DNA…

A: DNA fingerprint analysis consists of the process of identification of individuals based on their DNA…

Q: An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel,…

A: Lane 1 bottom band has below 500 basepairs. Lane 1 top band has 5000bp. Lane 2 band 1000bp.

Q: Restriction enzymes bind to specific sequences of DNA to seal them together. True False

A: Restriction enzymes bind to speffic sequence of DNA seal them together. This is TRUE. Restriction…

Q: Which of the following is most needed to determine the DNA quantity using the eyeball method? A DNA…

A: DNA quantity was determined by several methods where absorbance was one of the widely used methods…

Q: The concentration of a DNA sample is 40 nano grams per milliliter how many nanoliters will be needed…

A:

Q: When y

A: Introduction: Deoxyribonucleic Acid, or DNA, is a molecule that transports genetic data in all…

Q: DNA is charged and _fragments travel faster than positively, shorter, longer negatively, shorter,…

A: DNA is a polymer that is composed of two polynucleotide chains that coil around each other but…

Q: Which of the following is most needed to determine the DNA quantity using the eyeball method?  -DNA…

A: DNA ladder is the most needed thing to determine the DNA quantity using the eyeball method. As we…

Q: The technique that Rosalind Franklin used to take images of DNA is called NMR. O a. False O b. True

A: Nucleic acids are polynucleotides that contain many nucleotides that are bound by phosphodiester…

Q: In this gel, which letter (A, B, or C) indicates the largest piece of DNA in lane one? If the…

A: Gel Electrophoresis is a separation technique for the DNA fragments based upon their size. When the…

Q: If the primers you purchased possessed the following information. 5'-GGA AAC AGC TAT GAC CAT G-3'…

A: Introduction :- All living things require primers, which are brief, single-stranded nucleic acids,…

Q: True or false: During DNA isolation, ice cold 95% ethanol is used to digest the DNA into fragments…

A: During DNA isolation 95% ethanol is used for DNA precipitation not for DNA digestion and while we…

Q: DNA fragments can be separated by: O gel electrophoresis O autoradiography O x-ray crystallography O…

A: Phosphodiester linkages connect DNA strands, which are polymers of deoxynucleoside monophosphates.

Q: Make 2 mL of 50 fold dilution of DNA solution and sodium phosphate buffer.  DNA: 400 uL Sodium…

A: Dilution is the process of lowering the concentration of a solution by adding more of solvent to it.…

Q: Aside from the sample and assuming proper AGE protocol, which of the following is most needed to…

A: Agarose gel  electrophoresis is an important technique used in molecular biology research. In case…

Q: What is the size of the DNA sample based on the gel profile below? Make sure to indicate the units.…

A: Electrophoresis is the technique to separate biomolecules depending on their…

Q: 4. Determine the 260/280 ratios for each of the four samples. Record these values in Table 2.

A: Absorbance is the most common method to measure the purity of DNA yeild. This method is simple,…

Q: How much 6x loading dye should be added to 49 uL of DNA?  Report you answer in units of uL, with one…

A: The equation of dilution: M1xV1 = M2xV2 Where, M1 is the molar concentration of the stock solution.…

Q: put the follwoing steps in chrnological order based upon what you would need to do to deternine if…

A: DNA precipitation or determination of DNA in the organism is a complex process that involves the…

Q: Evaluate the gel provided below. Use the notes to help you read the gel: DNA is loaded in the wells…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Why does DNA appear when you add cold alcohol to the strawberry DNA solution?

A:

Q: Samples came to the laboratory for analysis. You are asked to identify the differences between these…

A: Spectrophotometer is used to determine the concentration of any unknown sample. DNA is a…

Q: 1 2 3 4 M 1000 b 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp 200 bp

A: The diagram is showing the separation of DNA fragments using gel electrophoresis.  In-gel…

Q: Which of the following conditions will affect the specificity of primer annealing? You may choose…

A: PCR stands for polymerase chain reaction, a technique used to make millions to billions of copies of…

Q: If you were to re-do this lab again with the same basic constraints (one DNA source, multiple…

A: DNA fragments are run on a agarose gel.

Q: How much 4x loading dye should be added to 11 uL of DNA?  Report you answer in units of uL, with one…

A: Recall the equation of dilution: C1 x V1 = C2 x V2 Where, C1 is the concentration of the stock…

Q: How would I make a calibration plot with gel electrophoresis? I LABLED THE SIDE WITH THE DNA…

A: In gel electrophoresis by applying an electric field to a gel matrix, DNA fragments migrate through…

Q: In which reagent is extracted DNA suspended to put it in solution? O Sodium dodecyl sulfate (SDS)…

A: Deoxyribonucleic acid is a polymer made up of two polynucleotide chains that coil around one another…

Q: Based on the result of spectrophotometry, what can you say about the quantity and purity of your DNA…

A: The spectrophotometer is a device that calculates how much light a substance absorbs. The…

Q: The following gel shows the results of a crime scene investigation. Lane 1 shows a DNA sample that…

A: Gel electrophoresis:- it is a technique used for the separation of Nucleic acids i.e. DNA and RNA as…

Q: Can a doctor make a plan for treatment for a person based solely on the information in the gel…

A: Gel electrophoresis is a laboratory technique for separating DNA, RNA, and protein mixtures based on…

Q: In lane 1, a size standard was loaded, which contained a mix a DNA fragments known to be 1000 bp,…

A: Gel electrophoresis is a gel technique that uses an applied electrical field to separate nucleic…

Q: I'm extracting my own DNA using a gargle salt mixed together with liquid detergent and gently pour…

A: DNA is a long chain of nucleotides that contain the complete biological genetic information of…

Crime Scene Investigation

A crime scene refers to a place where a crime has occurred. The purpose of crime scene investigation (CSI) is to analyze the complete scene and catch the culprit based on the pieces of evidence collected from the place of crime. The people involved in this process are referred to as crime scene investigators.