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Evaluate the gel provided below. Use the notes to help you read the gel: DNA is loaded in the wells (little pockets) at the top. The wells are the dark spaces under the numbers. DNA moves from the wells into the gel. The smaller the DNA the more quickly it moves. The samples on the ends are DNA ladders. We know the sizes of each of those bands and can use them to estimate the size of the DNA in the patient samples.

Evaluate the gel provided below. Use the notes to help you read the gel: DNA is loaded in the wells (little pockets) at the top. The wells are the dark spaces under the numbers. DNA moves from the wells into the gel. The smaller the DNA the more quickly it moves. The samples on the ends are DNA ladders. We know the sizes of each of those bands and can use them to estimate the size of the DNA in the patient samples.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Evaluate the gel provided below. Use the notes to help you read the gel: DNA is loaded in the wells (little pockets) at the top. The wells are the dark spaces under the numbers. DNA moves from the wells into the gel. The smaller the DNA the more quickly it moves. The samples on the ends are DNA ladders. We know the sizes of each of those bands and can use them to estimate the size of the DNA in the patient samples. 1 2 3 1: DNA ladder 2: Positive control 3: Patient 1 sample 4: Patient 2 sample 5: Patient 3 sample 6: Negative Control 7: DNA ladder 300 bp 100 bp 1. Why are positive and negative controls needed for PCR (or any test)? 2. Initially, test kits sent out by the CDC were flawed. The negative control sometimes showed up as positive. What misdiagnosis would potentially occur with this mistake? (Over or underdiagnosis?) 3. The CDC ultimately said to use the test anyway. Why might this be the case, given the current situation? 4. How would you evaluate the results above? Do any of the patients have SARS-CoV-2?
Transcribed Image Text:Evaluate the gel provided below. Use the notes to help you read the gel: DNA is loaded in the wells (little pockets) at the top. The wells are the dark spaces under the numbers. DNA moves from the wells into the gel. The smaller the DNA the more quickly it moves. The samples on the ends are DNA ladders. We know the sizes of each of those bands and can use them to estimate the size of the DNA in the patient samples. 1 2 3 1: DNA ladder 2: Positive control 3: Patient 1 sample 4: Patient 2 sample 5: Patient 3 sample 6: Negative Control 7: DNA ladder 300 bp 100 bp 1. Why are positive and negative controls needed for PCR (or any test)? 2. Initially, test kits sent out by the CDC were flawed. The negative control sometimes showed up as positive. What misdiagnosis would potentially occur with this mistake? (Over or underdiagnosis?) 3. The CDC ultimately said to use the test anyway. Why might this be the case, given the current situation? 4. How would you evaluate the results above? Do any of the patients have SARS-CoV-2?
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