You have 15 tubes, each containing 5 ul of DNA (each tube has a different DNA to cut). You want to cut all 15 with PstI. The easiest way to set up the digestions is to make a mix and add some to each tube.  Again, give ingredients and how much of each, and how much of the mix you would add to each tube. You will be using a 20 ul volume/tube. (Hint: DNA is NOT part of the mix, because each tube has a different DNA to cut, so you have to put that in separately. But the mix includes water, buffer, and enzyme.)

Q: Please convert to past tense and passive voice by using point form

A: The experimental procedure contains the specific techniques used in conducting a particular…

Q: In this activity you will extract DNA from living material. Before you begin, here are some helpful…

A: DNA (deoxyribonucleic acid) extraction is the process in which DNA of the given living organism or…

Q: A piece of linear DNA is 700000 base pairs in length and is digested with a restriction enzyme that…

A: Linear DNA is found in prokaryotes, which is linear in shape with two free ends. Restriction enzymes…

Q: Put a primer on the top and bottom of bubble at origin. Identify 3’ ends. You can use a thicker line…

A: For DNA replication to occur, following steps take place -

Q: You have 10 ul of DNA you want to cut with HindIII, and then run a gel, in order to cut out certain…

A: Restriction Digestion is a process by which DNA molecules are cut into small pieces with the aid of…

Q: This is a paper centrifuge: (you don't need to watch the whole video, just a few seconds to see how…

A: Centrifugal force is based on density gradient  In a centrifuge the densest object settle at the…

Q: you are given a printout of the sense strand of your DNA.  The printout is shown below. The first…

A: Introduction: DNA is a double helix having a sugar-phosphate backbone like a ladder and the base…

Q: Does each sample contain pure DNA? How do you know?

A: Not necessarily all the samples will have pure DNA it may have contaminations and the level of…

Q: Describe the principal functions of the two major groups of adreno-cortical steroids, the…

A: The adrenal cortex that forms the outer layer of the adrenal gland produces polycyclic steroid…

Q: (Practice Hint: Click on the right-hand column to choose a bar height. Click again to change the bar…

A: DNA is made up of four nitrogenous bases: adenine (A), thymine (T), guanine (G), and cytosine (C).…

Q: CT/TGT/AAG/ACC/TTT What would be the amino acid sequence created from this mutated DNA strand?

A: The central dogma theory in genetics is based on the processes of Replication, Transcription and…

Q: Consider the following image of a stained agarose gel representing the separation of a plece of DNA…

A: Gel electrophoresis is a biochemical technique in which macromolecules such as DNA  can be separated…

Q: The Alu 1 enzyme comes at a concentration of 5,000 U/ml. Its buffer comes 10X. The DNA is at a…

A: Given,- Alu 1 enzyme concentration: 5,000 U/ml- Alu 1 enzyme buffer concentration: 10X- DNA…

Q: You digest 4 uL of plasmid DNA that is 50 ng/uL concetration in a total volume of 20 uL. You run 10…

A: ANSWER) The amount of DNA digested can be calculated as follows: Total amount of DNA = volume x…

Q: When y

A: Introduction: Deoxyribonucleic Acid, or DNA, is a molecule that transports genetic data in all…

Q: A DNAR document is legally binding. True or False?

A:

Q: if You have been transported to a DNA Lab by a mad scientist.    The scientist hands you a gel…

A: DNA is one of the forms of nucleic acid and can be observed as a genetic material in most of the…

Q: You have a very concentrated sample of DNA which you need to dilute for sequencing 1:6000 with a…

A: Dilution is defined as the ratio of volume from stock to the final volume.  Dilution= Stock volume /…

Q: Restriction enzymes come in a concentration of U/ml, and it is recommended that 1U be used for each…

A: Restriction enzymes (RE) are the enzymes that recognize and cut specific sequences of DNA called…

Q: You will be setting up your diagnostic digest on three plasmids, the ones you began with, PGFPUV and…

A: In this question, we have to complete the table for the reaction mixture of a PCR experiment.

Q: How would you solve this and how would it look? i am confused

A: The objective of this question is to estimate the size of an unknown DNA fragment using a standard…

Q: Look at the amino acid chart below that the proctor placed on a board at the front of the classroom…

A: Sickle cell anemia is an inherited blood disorder that affects the structure and function of red…

Q: In your forensic laboratory, you set up a reaction to digest DNA with restriction enzymes. The final…

A: In molecular cloning techniques like PCR and restriction cloning, restriction enzyme digestion is…

Q: 1) The Nde1 enzyme comes at a concentration of 40 U/ul. According to the manufacturer, the container…

A: Question 1: Enzyme ConcentrationThe Nde 1 enzyme comes with a specified concentration of 40 U/μl.…

Q: 1). What is the concentration of a solution of DNA with an OD260 of 0.3? ----I got 15 micrograms/mL…

A: Values given  OD 260 = 0.3 A260 of 1.0 = 50µg/ml pure dsDNA Concentration (µg/ml) = A260 reading ×…

Q: The enzyme that removes the RNA primer from the Okazaki fragment is:   DNA pol III   DNA ligase…

A: Introduction: DNA is the hereditary material that defines every cell. It is found within the nucleus…

Q: You have the following DNA sequence: 5'GGT ACG TTG GGG CTC CAT3' This sequence is transcribed and…

A: “Since you have posted multiple questions, we will provide the solution only to the first question…

Q: There are 2 parts to this question: The following DNA strand (below) is about to undergo DNA…

A: The cellular functions are regulated/controlled by the DNA present within the nucleus of the cell.…

Q: Above are the results of gel electrophoresis following digestion with restriction enzymes. What is…

A: Using specialized enzymes known as restriction endonucleases, restriction digestion is a laboratory…

Q: c. Why the polar bodies cannot be fertilized by sperms?

A: Hi! Thanks for your question. But as you have posted multiple questions, I am answering only one for…

Nutrition and Digestion

The process of consuming nutrients to utilize them for various biological purposes is known as nutrition. The substances absorbed from food are known as nutrients. They serve different important functions in the body, such as repairing wounds and damages, generating energy for metabolism, and regulating biochemical reactions.