You have 15 tubes, each containing 5 ul of DNA (each tube has a different DNA to cut). You want to cut all 15 with PstI. The easiest way to set up the digestions is to make a mix and add some to each tube.  Again, give ingredients and how much of each, and how much of the mix you would add to each tube. You will be using a 20 ul volume/tube. (Hint: DNA is NOT part of the mix, because each tube has a different DNA to cut, so you have to put that in separately. But the mix includes water, buffer, and enzyme.)

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Nutrition and Digestion

The process of consuming nutrients to utilize them for various biological purposes is known as nutrition. The substances absorbed from food are known as nutrients. They serve different important functions in the body, such as repairing wounds and damages, generating energy for metabolism, and regulating biochemical reactions.