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Biochemistry
Please convert to past tense and passive voice by using point form

Please convert to past tense and passive voice by using point form

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Please convert to past tense and passive voice by using point form

Procedure: Each group receives ONE tube of double-stranded DNA sample, and 2 Eppendorf tubes of NaOH with 1.0M and 10.0M concentrations. Record the pH of both NaOH solutions. Steps: 1. Label three Eppendorf tubes with your group number. 2. Transfer 1 ml DNA sample to each of the Eppendorf tubes 1, 2 and 3. 3. Add 0.1 ml water to tube 1. 4. Add 0.1 ml 1.0M NaOH to tube 2. 5. Add 0.1 ml 10.0 M NaOH to tube 3. * BE CAUTIOUS! NaOH is highly corrosive and can cause irritation to eyes and skin. Wear gloves and lab coat. Handle alkali with care, and don't hold the samples at your level while adding alkali. 6. Pipet the mixture up and down five times slowly in each tube using 1 ml pipet tip to mix the samples. 7. Quickly transfer all content from tube 1 to cuvette. Record the absorbance of sample at 260nm (A260) using UV-Spectrophotometer at room temperature. Record the room temperature. 8. Repeat measuring the A260 of samples in tubes 2 and 3 at room temperature. 9. Transfer the samples back to Eppendorf tubes 1-3 respectively, cap the three Eppendorf tubes and heat them at 45°C in water bath for 10 minutes. Measure the A260 of samples from tubes 1-3 immediately. 10. Repeat Step 9 for other temperatures listed in the table below and measure the A260 immediately after 10 min incubation in water bath. 11. Cool down all Eppendorf tubes at room temperature for 10 minutes. Measure the A260 for the last reading.
Transcribed Image Text:Procedure: Each group receives ONE tube of double-stranded DNA sample, and 2 Eppendorf tubes of NaOH with 1.0M and 10.0M concentrations. Record the pH of both NaOH solutions. Steps: 1. Label three Eppendorf tubes with your group number. 2. Transfer 1 ml DNA sample to each of the Eppendorf tubes 1, 2 and 3. 3. Add 0.1 ml water to tube 1. 4. Add 0.1 ml 1.0M NaOH to tube 2. 5. Add 0.1 ml 10.0 M NaOH to tube 3. * BE CAUTIOUS! NaOH is highly corrosive and can cause irritation to eyes and skin. Wear gloves and lab coat. Handle alkali with care, and don't hold the samples at your level while adding alkali. 6. Pipet the mixture up and down five times slowly in each tube using 1 ml pipet tip to mix the samples. 7. Quickly transfer all content from tube 1 to cuvette. Record the absorbance of sample at 260nm (A260) using UV-Spectrophotometer at room temperature. Record the room temperature. 8. Repeat measuring the A260 of samples in tubes 2 and 3 at room temperature. 9. Transfer the samples back to Eppendorf tubes 1-3 respectively, cap the three Eppendorf tubes and heat them at 45°C in water bath for 10 minutes. Measure the A260 of samples from tubes 1-3 immediately. 10. Repeat Step 9 for other temperatures listed in the table below and measure the A260 immediately after 10 min incubation in water bath. 11. Cool down all Eppendorf tubes at room temperature for 10 minutes. Measure the A260 for the last reading.
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