step 4, isopropyl alcohol is used to precipitate out the DNA because it is not soluble. What does this mean? B. What role did the alcohol, detergent and salt play? C.
step 4, isopropyl alcohol is used to precipitate out the DNA because it is not soluble. What does this mean? B. What role did the alcohol, detergent and salt play? C.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
DNA Extraction Using household materials
- Wash the fruit.
- Place the fruit (e.g. banana) in a zip lock plastic bag and crush it with your fist.
- Add the one half teaspoon of salt and one tablespoon of dishwashing liquid to the mashed
fruit mixture in the bag, zip it up and squeeze it in your hands for 1 minute.
- Place the strainer over the small glass.
- Pour the mixture into the strainer. Filter the mixture into the glass.
- Slowly pour ice cold rubbing alcohol into the glass, until it is about half full.
- Let it stand until the alcohol will form a separate layer on top of the fruit filtrate.
- Keep the glass still at eye level; do not shake it. Watch what happens. DNA will begin to
appear where t 9. Carefully Scoop out the DNA with the straightened end of the paper clip.
- Touch the DNA. he alcohol and filtrate layer meet
A. In step 4, isopropyl alcohol is used to precipitate out the DNA because it is not soluble. What does this mean?
B. What role did the alcohol, detergent and salt play?
C.
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education