Homework Help is Here – Start Your Trial Now!
Science
Biology
РО P3mod 5' GAGTTTGATCMTGGCTCAG 3' .5' ATTAGATACCCTDGTAGTCC 3' Considering only the PO and the P3Mod primers from Table 1 in Wilson et al. (1990), complete the following table for PO and P3Mod to verify that these two primers adhere to the general criteria required for primer design: CHARACTERISTIC CRITERIA FOR AN EFFECTIVE PRIMER 1: PO PRIMER 2: P3MOD PRIMER Length of primer Minimum length 17 bases; between 17 - 30 bp 19 nt 20 nt Base composition G+C content 40-60% Tm of primers Tm 60-75°C The "Wallace rule" for calculating melting temperature TM: Tm = 2(A + T) + 4(G + C); where Tm is in °C and (A + T) is the sum of the A and T residues in the oligonucleotide and (G + C) is the sum of the G and C residues in the oligonucleotide.

РО P3mod 5' GAGTTTGATCMTGGCTCAG 3' .5' ATTAGATACCCTDGTAGTCC 3' Considering only the PO and the P3Mod primers from Table 1 in Wilson et al. (1990), complete the following table for PO and P3Mod to verify that these two primers adhere to the general criteria required for primer design: CHARACTERISTIC CRITERIA FOR AN EFFECTIVE PRIMER 1: PO PRIMER 2: P3MOD PRIMER Length of primer Minimum length 17 bases; between 17 - 30 bp 19 nt 20 nt Base composition G+C content 40-60% Tm of primers Tm 60-75°C The "Wallace rule" for calculating melting temperature TM: Tm = 2(A + T) + 4(G + C); where Tm is in °C and (A + T) is the sum of the A and T residues in the oligonucleotide and (G + C) is the sum of the G and C residues in the oligonucleotide.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question
РО P3mod 5' GAGTTTGATCMTGGCTCAG 3' .5' ATTAGATACCCTDGTAGTCC 3'
Transcribed Image Text:РО P3mod 5' GAGTTTGATCMTGGCTCAG 3' .5' ATTAGATACCCTDGTAGTCC 3'
Considering only the PO and the P3Mod primers from Table 1 in Wilson et al. (1990), complete the following table for PO and P3Mod to verify that these two primers adhere to the general criteria required for primer design: CHARACTERISTIC CRITERIA FOR AN EFFECTIVE PRIMER 1: PO PRIMER 2: P3MOD PRIMER Length of primer Minimum length 17 bases; between 17 - 30 bp 19 nt 20 nt Base composition G+C content 40-60% Tm of primers Tm 60-75°C The "Wallace rule" for calculating melting temperature TM: Tm = 2(A + T) + 4(G + C); where Tm is in °C and (A + T) is the sum of the A and T residues in the oligonucleotide and (G + C) is the sum of the G and C residues in the oligonucleotide.
Transcribed Image Text:Considering only the PO and the P3Mod primers from Table 1 in Wilson et al. (1990), complete the following table for PO and P3Mod to verify that these two primers adhere to the general criteria required for primer design: CHARACTERISTIC CRITERIA FOR AN EFFECTIVE PRIMER 1: PO PRIMER 2: P3MOD PRIMER Length of primer Minimum length 17 bases; between 17 - 30 bp 19 nt 20 nt Base composition G+C content 40-60% Tm of primers Tm 60-75°C The "Wallace rule" for calculating melting temperature TM: Tm = 2(A + T) + 4(G + C); where Tm is in °C and (A + T) is the sum of the A and T residues in the oligonucleotide and (G + C) is the sum of the G and C residues in the oligonucleotide.
Expert Solution
steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS