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РО P3mod 5' GAGTTTGATCMTGGCTCAG 3' .5' ATTAGATACCCTDGTAGTCC 3' Considering only the PO and the P3Mod primers from Table 1 in Wilson et al. (1990), complete the following table for PO and P3Mod to verify that these two primers adhere to the general criteria required for primer design: CHARACTERISTIC CRITERIA FOR AN EFFECTIVE PRIMER 1: PO PRIMER 2: P3MOD PRIMER Length of primer Minimum length 17 bases; between 17 - 30 bp 19 nt 20 nt Base composition G+C content 40-60% Tm of primers Tm 60-75°C The "Wallace rule" for calculating melting temperature TM: Tm = 2(A + T) + 4(G + C); where Tm is in °C and (A + T) is the sum of the A and T residues in the oligonucleotide and (G + C) is the sum of the G and C residues in the oligonucleotide.

РО P3mod 5' GAGTTTGATCMTGGCTCAG 3' .5' ATTAGATACCCTDGTAGTCC 3' Considering only the PO and the P3Mod primers from Table 1 in Wilson et al. (1990), complete the following table for PO and P3Mod to verify that these two primers adhere to the general criteria required for primer design: CHARACTERISTIC CRITERIA FOR AN EFFECTIVE PRIMER 1: PO PRIMER 2: P3MOD PRIMER Length of primer Minimum length 17 bases; between 17 - 30 bp 19 nt 20 nt Base composition G+C content 40-60% Tm of primers Tm 60-75°C The "Wallace rule" for calculating melting temperature TM: Tm = 2(A + T) + 4(G + C); where Tm is in °C and (A + T) is the sum of the A and T residues in the oligonucleotide and (G + C) is the sum of the G and C residues in the oligonucleotide.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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РО P3mod 5' GAGTTTGATCMTGGCTCAG 3' .5' ATTAGATACCCTDGTAGTCC 3'
Transcribed Image Text:РО P3mod 5' GAGTTTGATCMTGGCTCAG 3' .5' ATTAGATACCCTDGTAGTCC 3'
Considering only the PO and the P3Mod primers from Table 1 in Wilson et al. (1990), complete the following table for PO and P3Mod to verify that these two primers adhere to the general criteria required for primer design: CHARACTERISTIC CRITERIA FOR AN EFFECTIVE PRIMER 1: PO PRIMER 2: P3MOD PRIMER Length of primer Minimum length 17 bases; between 17 - 30 bp 19 nt 20 nt Base composition G+C content 40-60% Tm of primers Tm 60-75°C The "Wallace rule" for calculating melting temperature TM: Tm = 2(A + T) + 4(G + C); where Tm is in °C and (A + T) is the sum of the A and T residues in the oligonucleotide and (G + C) is the sum of the G and C residues in the oligonucleotide.
Transcribed Image Text:Considering only the PO and the P3Mod primers from Table 1 in Wilson et al. (1990), complete the following table for PO and P3Mod to verify that these two primers adhere to the general criteria required for primer design: CHARACTERISTIC CRITERIA FOR AN EFFECTIVE PRIMER 1: PO PRIMER 2: P3MOD PRIMER Length of primer Minimum length 17 bases; between 17 - 30 bp 19 nt 20 nt Base composition G+C content 40-60% Tm of primers Tm 60-75°C The "Wallace rule" for calculating melting temperature TM: Tm = 2(A + T) + 4(G + C); where Tm is in °C and (A + T) is the sum of the A and T residues in the oligonucleotide and (G + C) is the sum of the G and C residues in the oligonucleotide.
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