You perform a size exclusion chromatography experiment and generate the followingstandard curve.Kav10.830.60.40.201Your Answer:10Answery = -0.18ln(x) + 1.1100Molecular weightA test protein has a Kav of 0.40. What is this protein's molecular weight?Give your answer to the nearest whole number. Units and decimals are not needed.1000MacBook AirNII

In the question Kav vs molecular weight plot is given and the resulting linear equation is: y =…

Answered: You perform a size exclusion…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

A plaque assay is performed beginning with 1 mL of a solution containing bacteriophages. This solution is serially diluted 3 times by combining 0.1 mL of each sequential dilution with 9.9 mL of liquid medium. Then 0.1 mL of the final dilution is plated in the plaque assay and yields 12 plaques.What is the initial density of bacteriophages in the original 1 mL? Enter your answer to two significant figures ( for example: 1.1 * 10^2)
You are studying a protein-protein interaction in 2 proteins. You decide to test both these proteins in different buffers and see if the interaction is preserved. You run the assay in the following buffers, pH 7.4, pH 7.4 with BME, pH 4.5, pH 3.5, pH 11, pH 12.5. The SDS gels from the assays are attached. a. What type of protein-protein interaction do these results suggest? b. What amino acids are most likely involved in this interaction? (Guess based on assay)
You start with 100 units of protein activity and 100 grams of total protein. After the first centrifugation step, you have 25% less activity and 85% less total protein. What is your fold-purification of the target protein? 0.3-fold 3.4-fold 75-fold 5-fold 20-fold
You are studying a protein-protein interaction in 2 proteins. You decide to test both these proteins in different buffers and see if the interaction is preserved. You run the assay in the following buffers, pH 7.4, pH 7.4 with BME, pH 4.5, pH 3.5, pH 11, pH 12.5. The SDS gels from the assays are attached. a. What type of protein-protein interaction do these results suggest? b. What amino acids are most likely involved in this interaction?
13. Linear regression analysis was performed for the calibration standard data set based on this experiment. The analysis yielded the following results: m = 59.8805, b = -0.0042. What is the concentration of protein (mg/mL) in the original unknown protein solution if the measured absorbance is 0.736? The absorbance of a blank solution is 0.232. Assume a dilution factor of 40 for the analyzed unknown solution. 0.334 0.3225 0.339 0.3395
A packing plant fills bags with cement. The weight X kg of a bag of cement can be modelled by a Normal distribution with mean 50 kg and standard deviation of 2kg. a) If a bag is selected is at random, find the probability that the weight of the bag is greater than 55 kg. b) If three bags are selected at random, find the probability that 2 bags weigh more than 55 kg. c) Compute the probability in part a using RStudio.
You perform a Bradford assay. You obtain the absorbance values listed below from the BSA samples; your protein sample yields an absorbance of 1.3; what is the protein concentration of your sample? How did you determine that? BSA (ug/ml) Absorbance @ 595 nm. 25 0.15 50 0.30 75 0.45 100 0.60 150 0.90 200 1.25
In spectrophotometry, we measure the concentration of an analyte by its absorbance of light. A low concentration sample was prepared, and nine replicate measurements gave absorbances of: 0.0047, 0.0054, 0.0062, 0.0060, 0.0046, 0.0056, 0.0052, 0.0044, 0.0058. Nine reagent blanks gave values of: 0.0006, 0.0012, 0.0022, 0.0005, 0.0016, 0.0008, 0.0017, 0.0010, and 0.0011. a) What is the absorbance detection limit? This is related to both the scatter of the blank and the scatter in the measured samples.
Download BLOSUM30 and BLOSUMB0 substitu- tion matrices and place them side by side on your computer screen. What are the differences between the two matrices? Why do you see these differences?
Horizontal sequence :VIRL Vertical sequence:MKF Scoring rules: g/o = -3, g/e = -1, match or mismatch - from PAM250 substitution matrix below. NW algorithm. 1. Complete the scoring matrix. Scoring matrix with PAM250 scores: V I R L M K F 2. Set up, initialize and complete the NW matrix. 3. Retrace, align and score alignment(s). Use the arrows and circles for the matrix and path(s). V I R L M K F Align and score all optimal alignments here. PLZ the arrows and circles for the matrix and path(s) AND SHOW ALL possible Alignment
Please explain how to use the global alignment algorithm: Needleman-Wunsch. Use the match 3, mismatch -3, linear gap penalty -3. Protein sequence 1: PHWG Protein sequence 2: HWEG
use the values calculated (picture 2) to solve picture 1

SEE MORE QUESTIONS

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS