Enzyme chymotrypsin (enzyme digest) Samples: - 1 & 5- Standard Protein (Ladder) - 2 & 6- UNCUT (tube #1) - 3 & 7- 5 mins (tube #2) - 4 & 8- 30 mins (tube #3) Human and Bovine (Left) Chicken and Pigeon (Right) In this experiment we will be comparing the structural similarity of serum albumin from several different vertebrate species and thus examining how closely related the proteins from these species are to each other. These proteins have similar molecular weights. We will first cut the proteins with the enzyme chymotrypsin (enzyme digest). We will then separate the fragments based on their relative sizes through gel electrophoresis so that we can compare the fragments generated by the enzyme digest for the different species. Write the hypothesis and prediction for the experiment.
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
Enzyme chymotrypsin (enzyme digest)
Samples:
- 1 & 5- Standard Protein (Ladder)
- 2 & 6- UNCUT (tube #1)
- 3 & 7- 5 mins (tube #2)
- 4 & 8- 30 mins (tube #3)
Human and Bovine (Left)
Chicken and Pigeon (Right)
In this experiment we will be comparing the structural similarity of serum albumin from several different vertebrate species and thus examining how closely related the proteins from these species are to each other. These proteins have similar molecular weights. We will first cut the proteins with the enzyme chymotrypsin (enzyme digest). We will then separate the fragments based on their relative sizes through gel electrophoresis so that we can compare the fragments generated by the enzyme digest for the different species.
Write the hypothesis and prediction for the experiment.
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