You perform a Minimum Inhibitory Concentration (MIC) assay on a bacterium that you have isolated from a patient, with the results shown below (white material = bacterial growth). Which of the following is NOT a correct statement about these results? Erythromycin Gentamicin Concentration (ug/ml) 300 None of the other four answers (All statements are true 200 A lower concentration of erythromycin than gentamicin will inhibit growth of this bacterium The MIC for gentamicin was not determined in this test The MIC for erythromycin was 50ug/ml The bacterium was resistant to gentamicin at all concentrations tested
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- A further sample of blood was taken from the above individual and prepared for haemoglobin analysis. To 18 mls of blood, 2 mls of anticoagulant were added and 5 mls of this sample was centrifuged and a 2.6 ml pellet of red blood cells obtained. 10 mls of water were added and mixed to lyse the cells before a further centrifugation to remove cell membranes from the sample. 1 ml of this sample was further diluted by the addition of 5 mls of water to provide a stock sample of haemoglobin. What was the final dilution factor for this haemoglobin stock solution? O 1.16.76 QUESTION 3 О 2.1.67 О 3.1247 O 4.1.51 O 5.61.89You are performing an experiment to diagnose three patients who are ill. In a hematology lab, you run a CBC using the hematology analyzer on all 3 of your patience blood samples, once you collect that data, you then conduct a blood smear/stain to get a better look at the 3 patients blood cells and compare those results to those of the CBC report. You conclude that patient 1 has malaria, patient 2 has sickle cell anemia, and patient 3 has hemophilia. Can you Identify your independent, dependent and controlled variables?The physician ordered erythromycin 500 mg IV every 8 hours for infection. The pharmacy sends a vial labeled: Erythromycin 1 g. The nursing drug reference provides information to reconstitute 1 g of erythromycin with 20 mL of sterile water and further dilute in 250 mL of 0.9% NS and to infuse over 2 hours. How many ml will you draw from the vial after reconstitution and add it to the IV bag of 0.9% NS 250 mL? 20 mL 5 mL 15 mL 10 mL Page 10 of 14 Previous Page Next Page
- GenAlex Medical, a leading manufacturer of medical laboratory equipment, is designing a new automated system that can detect normal levels of dissolved immunoglobin A (70. to 312. mg/dL), using a blood sample that is as small as 250 µL. Calculate the minimum mass in milligrams of immunoglobin A that the new system must be able to detect. Be sure your answer has the correct number of significant digits. mg 0 x10 X1 ml of whole blood is collected and centrifuged to obtain a plasma sample. The volume of the plasma sample is 300 microlitres. A 1 in 4 dilution of the plasma is made and analysed using a cholesterol assay like the one you performed in the laboratory. 10 microlitres of both the diluted plasma sample and a cholesterol standard are assayed using 1mL of cholesterol reagent. Following incubation at 37 degrees Celcius, the samples are measured spectrophotometrically at 500nm. If the absorbance of the plasma sample assay was 0.5, the absorbance of the cholesterol standard assay was 0.4, and the concentration of cholesterol in the standard was 1.3mM, what was the concentration of the cholesterol in the whole blood (in mM)? (assume all of the cholesterol in the whole blood sample was recovered in the plasma; do not include units in your answer; give your answer to 2 decimal places)You are given a cell stock A. You make a 1:100 dilution and load 10uL diluted sample to a hemocytometer. After counting all 5 "one millimeter squares" you have 51 cells in average. (Please write down each step)T 1. What is the cell concentration of this diluted sample? 2. What is the cell concentration of original cell stock ? 3. If the original stock A has a total volume of 5mL, what is total number of cells in the A? 4. You decide to do a 1:1000 dilution to verify your counting. After loading and counting, you find out that you have 20 cells in average in each square. Do you think the new concentration is consistent with your previous result? If not, list all the possible errors that can cause this inconsistency. MacBook Air
- The figures below are the results of flow cytometer analysis of a drugs (camptotechin) effect on cells? From what you learned in class about flowcytometry, briefly state what the drug is doing to the cells: HL60 Untreated Camptothecin (45um, 2h) Camptothecin (45um, 4h) 3.36 10 PI (FL2)A White blood cell Suspension is dyed by Trypan Blue in 1:1 Ratio(Volume). 7 square is counted and the values are; Total Viable Cells: 168 Total Nonviable Cells:32 a.Percentage of Viable Cells: ? b.Average Number Of cells per square: ? c.Dilution Factor:? d.Concentration (Viable Cells/ml): ?The order is for Streptomycin 1gm IM. You have a 5gm vial of Streptomycin . The label states to add 9ml of sterile water to yield 400mq / m * l How many ml will you give?
- Hello, i'm having a hard time organzing the calculations when it comes to rate mL/h. I already solved this problem but I need your help to confirm if my calculations were correct. I would appreciate if you can add step by steps in order for me to understand it better, thank you! Order: Pulmocare 480 mg daily via PEG over 6 hours, followed by 50 mL sterile water flush. 1. The Pulmocare label states 100 mg/100 mL. At what rate in mL/h will you set the feeding pump to administer the Pulmocare? 2. Calculate the total amount of enteral fluid the patient will receive in 24h 3. How many milliliters of IV fluids is the patient receiving in 24h?The figure above depicts an agar cube with a side length of 13\, \text{mm}13mm13, start text, m, m, end text. In an experiment, students submerged the cube in red dye for 121212 hours. The red dye permeated 1\, \text{mm}1mm1, start text, m, m, end text on each side, as indicated by the shading in the figure. Volume of a rectangular solid: V = lwhV=lwhV, equals, l, w, h Calculate the volume of the agar cube that remained unpenetrated by the red dye.A client has been ordered methyl Pprednisulune 30omg luPB In 30mL D5W Over 15 min.stat fur an asthma att-acis. in Stous,you have methylpredrisolune 500mg vials . The reconstitutiun Instructions are: Add 7.8mL G Sterile uater and shale genly Each reconstituted uial contains 8mL. a) Houw nmuch medieation will you draw up? 6) what is the Infusion rate in mt/ hr ?