Samples: - 1 & 5: Standard proteins (Ladder) - 2 &6: Uncut - 3 & 7: 5 mins - 4 & 8: 30 mins Human and Bovine (Left) Chicken and Pigeon (Right) What do you expect in the lane for #1 tubes? Explain the reason for your expectation. Do the results match the expectations? Do you observe differences in the lanes for tubes 2 and 3? What does that tell you? Are there differences among the species? What does that tell you about how closely related the proteins are from these species? Do the results match the prediction and support the hypothesis? How would your results have been different if an enzyme with a different amino acid sequence preference had been used?
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
Samples:
- 1 & 5: Standard proteins (Ladder)
- 2 &6: Uncut
- 3 & 7: 5 mins
- 4 & 8: 30 mins
Human and Bovine (Left)
Chicken and Pigeon (Right)
- What do you expect in the lane for #1 tubes? Explain the reason for your expectation. Do the results match the expectations?
- Do you observe differences in the lanes for tubes 2 and 3? What does that tell you?
- Are there differences among the species? What does that tell you about how closely related the proteins are from these species? Do the results match the prediction and support the hypothesis?
- How would your results have been different if an enzyme with a different amino acid sequence preference had been used?
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