rom the MW analysis; are any of the gel and immune-detected bands consistent with what you expect for IgG? Are the gel and immune-detected bands suspected to be IgG consistent in distance migrated with to with respect to the molecular ladder? What is the difference between the whole calf serum and the “purified” lanes?  Were we able to truly purify IgG with the magnetic bead protocol?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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From the MW analysis; are any of the gel and immune-detected bands consistent with what you expect for IgG? Are the gel and immune-detected bands suspected to be IgG consistent in distance migrated with to with respect to the molecular ladder? What is the difference between the whole calf serum and the “purified” lanes?  Were we able to truly purify IgG with the magnetic bead protocol?

8
9
10
250-
250
150
-150
100
100
75
-75
Heavy Chain
50
37
Light Chain
25
-25
Std
Protein Standard
IgG
Elution
Unbound
Wash
Bovine Calf Serum
Elution
Unbound
Wash
6X LD
Transcribed Image Text:8 9 10 250- 250 150 -150 100 100 75 -75 Heavy Chain 50 37 Light Chain 25 -25 Std Protein Standard IgG Elution Unbound Wash Bovine Calf Serum Elution Unbound Wash 6X LD
Std
Protein Standard
IgG
Elution
Unbound
4-
Wash
5
Calf Serum
Elution
Unbound
Wash
ö 6X LD
Transcribed Image Text:Std Protein Standard IgG Elution Unbound 4- Wash 5 Calf Serum Elution Unbound Wash ö 6X LD
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