Please help me answer and explain these questions: 1. Humans also express serum albumin. How do the predicted molar extinction coefficient and molecular mass of human and bovine serum albumin compare? 2. Which assay (A280 or Bradford) can sense lower concentrations of protein? What is the LOD for each assay?
Please help me answer and explain these questions:
1. Humans also express serum albumin. How do the predicted molar extinction coefficient and molecular mass of human and bovine serum albumin compare?
2. Which assay (A280 or Bradford) can sense lower concentrations of protein? What is the LOD for each assay?
1&2)
The molar extinction coefficient and molecular mass of proteins can be used to quantify and identify proteins in a sample. In this context, two proteins of interest are human and bovine serum albumin. These proteins are members of the albumin family and share a high degree of sequence homology. In addition to their structural similarities, it is also important to compare the molar extinction coefficient and molecular mass of these proteins to better understand their behavior and properties.
In order to measure protein concentrations, two assays are commonly used: the A280 assay and the Bradford assay. The A280 assay is based on the absorbance of proteins at 280 nm, while the Bradford assay is based on the binding of proteins to the dye Coomassie Brilliant Blue. Both assays have different sensitivities and limitations, with the Bradford assay being more sensitive and able to detect lower concentrations of protein. The limit of detection (LOD) for each assay is also an important factor to consider when choosing an assay to use.
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