After adding ammonium sulfate, the mixture was allowed to stand with occasional stirring for 30 minutes in an ice bath. The formation of a white precipitate is expected to happen QUESTION: Explain the significance of allowing the mixture to be submerged in an ice bath for a given time.
Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation (Salting Out)
The computed amount of powdered ammonium sulfate was added to the egg white sample portion by
portion with constant stirring while submerged in an ice bath. The solution is expected to become more
turbid, and a white precipitate is expected to form.
The resulting mixture was then filtered using a cheesecloth. The residue was discarded, and 30.0 mL of the
filtrate was brought from 40% to 60% saturation by adding the required amount of powdered ammonium
sulfate in the same manner as the previous addition.
After adding ammonium sulfate, the mixture was allowed to stand with occasional stirring for 30 minutes in
an ice bath. The formation of a white precipitate is expected to happen
QUESTION:
Explain the significance of allowing the mixture to be submerged in an ice bath for a given time.
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