Separation of Amino Acids by Thin Layer Chromatography Lab Questions 7. Why must a pencil be used for drawing the line and spotting? 8. How will the Rf value of a compound be affected if the developing solvent is allowed to run off the top of the TLC plate (e.g. the TLC plate is allowed to remain in the developing chamber after the solvent front has reached the top of the plate.)? Explain. Be specific. 9. Describe what an iodine vapor chamber is and explain how does it work? (Hint: Background information – showing spots up chemically talks about two methods using Ninhydrin and Iodine crystals)
Separation of Amino Acids by Thin Layer Chromatography Lab Questions 7. Why must a pencil be used for drawing the line and spotting? 8. How will the Rf value of a compound be affected if the developing solvent is allowed to run off the top of the TLC plate (e.g. the TLC plate is allowed to remain in the developing chamber after the solvent front has reached the top of the plate.)? Explain. Be specific. 9. Describe what an iodine vapor chamber is and explain how does it work? (Hint: Background information – showing spots up chemically talks about two methods using Ninhydrin and Iodine crystals)
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Separation of Amino Acids by Thin Layer Chromatography Lab Questions
7. Why must a pencil be used for drawing the line and spotting?
8. How will the Rf value of a compound be affected if the developing solvent is allowed to run off the
top of the TLC plate (e.g. the TLC plate is allowed to remain in the developing chamber after the
solvent front has reached the top of the plate.)? Explain. Be specific.
9. Describe what an iodine vapor chamber is and explain how does it work? (Hint: Background
information – showing spots up chemically talks about two methods using Ninhydrin and Iodine
crystals)
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