B. Use the following table to answer the questions related to the Biuret protein test (5 - 7): Tube Solution Reaccion of color 1 2 mL egg albumin Violet 2 2 mL honey blue 3 2 mL solution of amino acid Violet 4 2 mL destilled water blue 5 2 mL solution of protein Violet 5. In the Biuret test, which of the solutions is a negative control? Positive? 6. Which contains more protein (C-N bonds): egg albumin or honey? Explain. 7. Do free amino acids have peptide bonds?
Hi. Based on the table i need to answer the questions. The table doesnt need to be filled.
|
Tube |
Solution |
Reaccion of color Benedict |
Reaccion of color mud |
| x | x | x | x |
| x | x | x | x |
| x | x | x | x |
| x | x | x | x |
| x | x | x | x |
| x | x | x | x |
|
7 |
10 drops of starch solution |
blue |
Ambar |
4. In the starch iodine test, which solution is a negative control? Positive?
B. Use the following table to answer the questions related to the Biuret protein test (5 - 7):
|
Tube |
Solution |
Reaccion of color |
|
1 |
2 mL egg albumin |
Violet |
|
2 |
2 mL honey |
blue |
|
3 |
2 mL solution of amino acid |
Violet |
|
4 |
2 mL destilled water |
blue |
|
5 |
2 mL solution of protein |
Violet |
5. In the Biuret test, which of the solutions is a negative control? Positive?
6. Which contains more protein (C-N bonds): egg albumin or honey? Explain.
7. Do free amino acids have peptide bonds?
C. Answer the following questions:
8. Why does the methylene blue dye stain the nuclei in onion cells?
9. What is the cause of the variation in color intensity in a biochemical test?
10. You have negative results on both the Benedict and Biuret tests but both look blue. Why?
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