4. Answer the following questions about dilutions. b You take a culture of bacteria and dilute it 5 times. Each dilution uses 1 ml of culture and 9 mi of saline solution. You take.Imi from the most dilute tube and plate it on a TSA plate. The next day you count 61 colonies. What was the original concentration 6.1X10³ CU ON 61 X 10² CFU 61 X 10² CFU 6.1 X 10² CFU/ You have a culture that has 5x10 cfull of bacteria in it. You performa serial dilution by adding 1 mi of the stock in Pi of TSB in Tube A and mix You then take 1 ml from Tube A and add it to 9 ml of TSB in Tube Band Last you take 1 from tube 8 and add it to a TSA plate. After overnight incubation, you count the colonies you see. How many colonies will you see? 50 Oas You are doing a series of serial dilutions to find the concentration of bacteria in CFU/ml in a culture. You plate several of the dilutions tubes and count the colonies the next day. On plate A you find over 1000 colonies, plate B you find 157 colonies and plate C you find 7 colonies. Which plate(s) can be used to determine the concentration in the original tube? Plate B None of the plates can be used Od Plates A. B and C Plate C el Plate A
4. Answer the following questions about dilutions. b You take a culture of bacteria and dilute it 5 times. Each dilution uses 1 ml of culture and 9 mi of saline solution. You take.Imi from the most dilute tube and plate it on a TSA plate. The next day you count 61 colonies. What was the original concentration 6.1X10³ CU ON 61 X 10² CFU 61 X 10² CFU 6.1 X 10² CFU/ You have a culture that has 5x10 cfull of bacteria in it. You performa serial dilution by adding 1 mi of the stock in Pi of TSB in Tube A and mix You then take 1 ml from Tube A and add it to 9 ml of TSB in Tube Band Last you take 1 from tube 8 and add it to a TSA plate. After overnight incubation, you count the colonies you see. How many colonies will you see? 50 Oas You are doing a series of serial dilutions to find the concentration of bacteria in CFU/ml in a culture. You plate several of the dilutions tubes and count the colonies the next day. On plate A you find over 1000 colonies, plate B you find 157 colonies and plate C you find 7 colonies. Which plate(s) can be used to determine the concentration in the original tube? Plate B None of the plates can be used Od Plates A. B and C Plate C el Plate A
Chapter9: Parenteral Medication Labels And Dosage Calculation
Section: Chapter Questions
Problem 1.1P
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Transcribed Image Text:4. Answer the following questions about dilutions.
a.
You take a culture of bacteria and dilute it 5 times. Each dilution uses 1 ml of culture and 9 ml of saline solution. You take 1 ml from the most dilute tube and plate it on a TSA plate. The next day you count 61 colonies. What was the original concentration?
- ○ 6.1 x 10⁶ CFU/ml
- ○ 6.1 x 10⁵ CFU/ml
- ○ 4.1 x 10⁶ CFU/ml
- ○ 4.1 x 10⁵ CFU/ml
b.
You have a culture that has 5 x 10³ CFU/ml of bacteria in it. You perform a serial dilution by adding 1 ml of the stock in 9 ml of TSB in Tube A and mix. You then take 1 ml from Tube A and add it to 9 ml of TSB in Tube B and mix. Last, you take 1 ml from Tube B and add it to a TSA plate. After overnight incubation, you count the colonies you see. How many colonies will you see?
- ○ 3000
- ○ 500
- ○ 50
- ○ 5
- ○ ≤ 5
c.
You are doing a series of serial dilutions to find the concentration of bacteria in CFU/ml in a culture. You plate several of the dilutions tubes and count the colonies the next day. On plate A you find over 1000 colonies, plate B you find 157 colonies, and plate C you find 7 colonies. Which plate(s) can be used to determine the concentration in the original tube?
- ○ Plate B
- ○ None of the plates can be used
- ○ Plates A, B and C
- ○ Plate C
- ○ Plate A
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