9. You make a 2 ml starter culture of a strain of E coli you typically work with. You take 100 µl of this starter culture and inoculate flask containing 1 liter of TSA broth and incubate it for 20 hours. In the meantime you take 10 µl of this culture and do serial dilutions to determine the number of cells that you inoculated the large culture with. What is the concentration of E coli? 10 μι PES 50 ul 50 ul 50 ul 50 μ 50 ul 450 ul PBS 45P ul PBS 450 ul PBS 450 ul PBS 450 ul PBS

Q: One ml of a water sample was placed in a Petri dish with melted agar. After incubation, 129 colonies…

A: A colony-forming unit, or CFU, is a measurement of the concentration of microorganisms in a test…

Q: How would you prepare a 10-7dilution from a culture? Each of your dilution tubes cannot hold more…

A: Dilution is the process of lowering a solute's concentration in a solution, typically by mixing it…

Q: If a culture of E. coli you are tracking grows from 5 x 103 cells/ml to 9 x 107 cells per ml in 4…

A: Cell culture refers to the in vitro growth and maintenance of cells, typically of animal or plant…

Q: Based on the following image, which concentration of antibiotic would be considered the minimum…

A: Minimum bactericidal concentration (MBC) is defined as the lowest concentration in which an…

Q: What results on a microdilution antimicrobial susceptibility test would indicate an antibiotic would…

A: An antimicrobial susceptibility test is a scientific procedure used to evaluate how effective…

Q: You have a culture that has 5 X103 cfu/ml of bacteria in it. You perform a serial dilution by adding…

A: In microbiology and bacteriology, the technique of serial dilution is a fundamental method used to…

Q: transferred to the plate. After 24 hours there were 60 colonies on the plate. What is the dilution…

A: b. The dilution factor is 1000. The patient sample contains 600,000 cells/ml

Q: 6a. A sample is given to you that contains 6 million cells per ml.  You must make a series of serial…

A: A log dilution is a tenfold dilution, meaning the concentration is decreased by a multiple of ten.

Q: 1. You have a culture of yeast that is at a density of 8x10' cells/mL 2. A lab technician took a…

A: Serial dilution is a process of diluting the stock sample to decrease its concentration by serially…

Q: You are working in a medical lab and receive a urine sample from a patient that is displaying…

A:

Q: The PD# of your culture is 165. You plated 3745 cells per well. You have recovered 12,655 cells.…

A: The passage number of a cell culture is a record of the number of times the culture has been…

Q: Questions 1 Predict the effect of the following "mistakes" made when performing a Gram stain. Your…

A: In order to understand the mistakes made in Gram staining procedure we need to understand the whole…

Q: Solution Stock solution Working solution Volume needed restriction digestion buffer 10X DNA 200…

A: In order to set up restriction digestion, we must mix precise amounts of various components to get…

Q: You prepared 10-7, 10-8, and 10-9 dilutions of a bacterial suspension in sterile saline. Then you…

A: Introduction There are different culturing techniques such as agar plates, broth culture, stab…

Q: 1. TRUE or FALSE. Relative to a 24 hour culture of endospore forming bacteria, a 72 hour culture…

A: Relative to a 24 hour culture of endospore forming bacteria, a 72 hour culture would contain more…

Q: Why do microbiologists use cultured plates with 30 to 300 colonies used for calculations?  2. Why…

A: 1. Microbiologists generally used cultured plates with 30 to 300 Colonies for calculation purposes.…

Q: You want to create a 10-fold dilution . You have a saline tube with a volume of 450ul. How much of…

A: According to this theory, a diluted solution's volume will be 10 times greater than its stock…

Q: Dilute cells from an old culture 1:200 into 5 mL of fresh media. What volume of the old culture…

A: Introduction Dilution is the process of lowering the concentration of a solute in a solution by…

Q: Which of the following are true of the above picture? (Select all that apply.) V The bacteria are…

A: Gram-negative bacteria have a thin peptidoglycan layer which fails to retain colour after treatment…

Q: Each Escherichia coli cell weighs 5ng. How many cells are present in a colony that weighs 1mg?

A: E. coli is a rod-shaped, Gram-negative, facultative anaerobic coliform bacteria of the genus…

Q: To determine if the type of agar affects bacterial growth, a scientist cultures on fou different…

A: Agar is galactose based heterogeneous polysaccharide. Agar is made from red algae. The main…

Q: A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine…

A: To keep the number of colonies on the plate within a certain range, serial dilutions of culture are…

Q: You obtain a 1 mL sample of contaminated drinking water in order to calculate the number of  fecal…

A: The evaluation of fecal microscopic organisms in water could be a critical public well-being measure…

Q: 3). The diagram below shows growth of E. coli strains 1 through 4 on a variety of media. Small dots…

A: Auxotrophy is the inability of an organism to synthesize a particular biochemical compound required…

Q: colony-forming units

A: Colony Forming Unit:It is designated as CFU.It can defined as the unit of measurement used to…

Q: B D F A с E Shape Flagella Staphylococc Bacillus Staphylococci Bacillus Diplobacillus Gram stain…

A: Introduction R. H. Whittaker’s classification system divides organisms into five major groups. These…

Modes of Nutrition

It is a biological process of providing or obtaining food that is necessary for growth and health. It includes many physiological processes - absorption, ingestion, assimilation, catabolism, excretion, etc. In biology, the science that deals with nutrition's physiological and chemical processes are called "nutritional science."