6a. A sample is given to you that contains 6 million cells per ml. You must make a series of serial dilutions in order to get a countable number on a plate to prove that there are indeed 6 million cells per ml. The plate that you eventually put a sample of bacteria on must have between 10 and 100 colonies for ease of counting. What dilutions do you make to get this many colonies on a plate? How many colonies do you have on that plate? Show what dilutions you make (Write exactly how much liquid you move into each test tube and how much liquid was in the test tube, ex. Move 1 ml of culture into 9 ml of water – 10x dilution). Don’t forget to tell me how much liquid you eventually put on your plate. (If you want to draw something out, you could draw then take a picture of it with your phone and submit the image along with the worksheet). 6b. Do the same problem as in 6a, but now you have to do it in 3 test tubes or less. What dilutions do you do now?
6a. A sample is given to you that contains 6 million cells per ml. You must make a series of serial dilutions in order to get a countable number on a plate to prove that there are indeed 6 million cells per ml. The plate that you eventually put a sample of bacteria on must have between 10 and 100 colonies for ease of counting. What dilutions do you make to get this many colonies on a plate? How many colonies do you have on that plate? Show what dilutions you make (Write exactly how much liquid you move into each test tube and how much liquid was in the test tube, ex. Move 1 ml of culture into 9 ml of water – 10x dilution). Don’t forget to tell me how much liquid you eventually put on your plate. (If you want to draw something out, you could draw then take a picture of it with your phone and submit the image along with the worksheet).
6b. Do the same problem as in 6a, but now you have to do it in 3 test tubes or less. What dilutions do you do now?
please use drawings to help explain. thank you.
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