After completion of a Gram stain procedure, you notice the bacteria cells shown in the picturebelow. How would you categorize the bacteria based on 1. Gram stain results, 2. Cellarrangement, and 3. cell shape.?Would the bacteria in question 7 have a thick layer of peptidoglycan or a thin layer ofpeptidoglycan in its cell wall?Would the bacteria in question 7 have an outer lipopolysaccharide layer in its cell wall?Would the antibiotic penicillin likely work to eliminate infection with the bacteria in question 7?How would you describe the type of flagella arrangement for the bacterium below? (You'll useone word for the description here.)

Gram stain results, cell arrangement, and cell shape:Gram stain results: The bacteria appear purple,…

Answered: After completion of a Gram stain…

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing

Problem 2RQ

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In the Gram stain, gram-negative bacteria appear red for all the following reasons EXCEPT: They stain with safranin because they are colorless at the step where it is added Safranin is a red stain None of the other four answers (all are true) The peptidoglycan layer in their cell walls is too thin to retain the crystal violet-iodine complex when the decolorizer (alcohol) is added The gram-negative cells' outer lipopolysaccharide layer (outer membrane) prevents their being stained by crystal violet in the first step
What are the structural differences between Gram-positive and Gram-negative bacteria cell walls? Can you explain what would happen to the cell wall's physical integrity while applying the Gram staining technique?
Identify the cellular morphology for this Gram stain. What is the Gram reaction? What is the bacterial shape? What is the bacterial arrangement? What is the staining dye? [Choose ] [Choose ] Cocci Singles no arrangement Pleomorphic Chains Gram-negative Crystal Violet Phenol Red Bacilli Gram-positive Clusters Safranin
When performing a Gram stain on a Gram-negative bacteria, why does the bacteria stain pink? Crystal violet stain binds to iodine and remains in the bacterial cell wall because of the thick peptidoglycan layer. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. The alcohol wash removes crystal violet and iodine from the bacteria. Crystal violet stain binds to iodine and remains in the bacteria cell wall because of the thick peptidoglycan layer AND Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink AND The alcohol wash removes crystal violet and iodine from the bacteria.
Examine the image below. The bacteria in this image have been treated with gram straining procedure. Indicate the following information A. Shape ........; Gram stain ( Gram (+), Gram (-) or both ... B. Stained color under microscope..... . C. Is the PG wall think, thin or both ..
Contrast the overall cell wall compositions of Gram positive and Gram negative bacteria, and go step-by-step in the procedure of the Gram stain. Describe what is occurring during each step within both Gram-positive cells and in Gram-negative cells. Be sure to include the appearance of each cell type at the end of each step.
What is the process to perform a Gram Stain? What happens if you make a mistake on a step (Think about different mistakes you could make)? What are the proper results? What structures do Archaeans use to attach to surfaces (Look like treble hooks)? What are bacterial inclusions? What are the similarities and differences in Gram - cells and Gram + cell structure? What is the function of ribosomes and what are they mad up of? What component of bacterial cells helps to combat/regulate osmotic forces? What are Koch’s postulates? What are they used for? Explain the process of endospore formation in endospore-producing organisms. What are the major components of the bacterial & eukaryotic cell? What is/are a pilus/pili and what do microbes use them for? What is the prokaryotic flagellum made up of?
In lab, we used the Gram stain to differentiate between Gram-positive and Gram-negative bacteria. Some bacteria stain purple (retaining the crystal violet) while others stain pink (from the counter-stain safranin). a) What is the biological molecule that is present in these bacteria that produces different results between Gram-positive and Gram-negative bacteria (be specific!) AND b) What is the difference between Gram-positive and Gram-negative bacteria that creates the different staining patterns (purple vs. pink)? (2-3 sentences max)
Identify the shapes and arrangements of the following bacteria observed under the microscope. Recall: look for the arrangement seen with most cells; you may see another arrangement that is less common. option: diplo, bacillus, negative, coccus, positive, staphylo, strepto. Gram stain Cell arrangement Cell Shape
Several students argued about why Gram-positive cells stain purple with the Gram stain whereas Gram-negative cells stain pink. Which explanation is correct? Multiple Choice Alcohol dehydrates peptidoglycan; the thick layer of dehydrated peptidoglycan in Gram-negative cells prevents the crystal violet from being washed out Safranin stains lipopolysaccharide, and because only Gram-negative cells have lipopolysaccharide, those cells look pink. Safranin stains lipopolysaccharide, and because only Gram-negative cells have lipopolysaccharide, those cells look pink. Alcohol dehydrates peptidoglycan; the thick layer of dehydrated peptidoglycan in Gram-positive cells prevents the crystal violet from being washed out. Crystal violet specifically stains peptidoglycan, and because Gram-positive cells have a thicker peptidoglycan layer those cells look purple. Alcohol destroys lipopolysaccharide in Gram-negative cell walls, allowing safranin to enter the cells and staining them pink.
Describe in detail all the steps needed to determine whether an Unknown bacteria has a Gram-positive or Gram-negative cell wall using the Gram stain. (You can describe the method presented in the Exercise 3-6 Gram stain videos or in the Lab Manual.) 1. Start by describing the method of transferring the bacteria onto the microscope slide, then describe heat-fixing the emulsion of the Unknown bacteria. (Do not describe all the steps needed to perform aseptic technique. Just say “Bacteria was added aseptically.”) 2. Describe the Gram stain in detail. (This description should include all the stains, how long the stain should set on slide, and Genus and species names of bacteria you are using as CONTROLS.)
Why do you suppose endospore-forming bacteria are commonly found in the soil? Suppose your unknown bacteria is a Gram-negative rod. You decide to perform an endospore stain, thinking it will help you with the identification of the organism. What will you see, and why?

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