The bacterial cells in the image below were stained with a high concentration of carbolfuschin for an extended period of time (5 minutes) while the slide was heated. The slide was decolorized with a mixture of acid and alcohol. The slide was then counter-stained with methylene blue.   What kind of stain does this describe?

Q: Peptidoglycan on bacterial cell walls is stained by crystal violet dye and for this reason those…

A: True

Q: layer

A: The crystal violet eventually escapes from cytoplasm of the cell, if left too long for…

Q: Describe in detail all the steps needed to determine whether an Unknown bacteria has a Gram-positive…

A: Introduction: Gram staining is a method developed by Danish bacteriologist Hans Christian Gram in…

Q: what color would you expect for a gram positive microorganism for each incorrect mistakes while…

A: "Gram staining" is a technique used in microbiology. It is a method in which a bacterium can be…

Q: If these cells are actually Gram +, which of the following likely occurred during the staining…

A: The Gram's method may be a standard laboratory method wont to divide bacterial species into two…

Q: As part of an experiment where absorbance values are measured using a spectrophotometer, you are…

A: The bacteria growth uses mitotic cell divisions. The bacterial population grows until the nutrients…

Q: An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and…

A: Introduction: Acid-fast staining is a type of differential staining technique that was first…

Q: Basic stains are more effective for bacterial staining than acidic dyes. Why? Which component on the…

A: Answer 1)Basic stains , which carries the postive charge chromogen are preferred because the…

Q: The KD of a given objective lens is 1.5 μm/ocular unit, a microbe spans 3 ocular units. What is the…

A: An objective lens is a lens system that is typically found on microscopes and other optical devices,…

Q: Is the flagellar stain a Positive or Negative Simple Stain protocol?

A: Staining is a technique that is used to enhance contrast in samples, generally at the microscopic…

Q: Move the images of bacterial cells into the chart to illustrate the color of gram-positive and…

A: Gram staining is a technique used to differentiate between two groups of bacteria namely…

Q: Acid-Fast stain M. Smegmatis -morphology shape -Arrangement -Acid-fast or non-acid-fast

A: Introduction :- Mycobacterium smegmatis is a rod-shaped bacterium that is commonly used as a model…

Q: How are negative stains prepared?Name two applications for which this type of stain especially…

A: Negative staining is defined as a method of demonstrating the type of small objects (like bacteria)…

Q: Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct)   a…

A: Simple stains- A stain that employs a single stain to color a bacteria: they are quick and easy.…

Q: Is acid fast staining differential stain , if yes, what are the 2 types of stain used

A: Differential Staining is defined as a staining process which utilizes more than one chemical stain.…

Q: Is is best to observe slides of bacteria stained with the acid fast stain under high dry…

A: The acid-fast stain is used to stain the acid-fast organisms. Acid-fast microorganisms have a thick…

Q: After performing the gram stain you observe your result as gram negative. To help confirm this…

A: Gram staining is a technique used for staining the microbial samples. It was performed by the…

Q: The acid-fast stain is used to stain _____. The decolorizer used to destain the cells is ______.…

A: The staining procedures used to differentiate the types of bacteria are known as a differential…

Q: What step normally associated with staining bacterial cells is omitted when the dimensions of cells…

A: Step 1 Gram staining is a technique of staining that is used to differentiate and categorize…

Q: Short Essay: Answer the prompt in 4 sentances or less. Describe the staining technique used to…

A: Staining is a protocol used to visualize cells/ tissues under a microscope. Staining colors and…

Q: You mistakenly confused the primary stain and counter stain with crystal violet, how does you…

A: Gram staining is a widely used laboratory technique in microbiology and bacteriology to…

Q: Examine the image below. The bacteria in this image have been treated with gram straining procedure.…

A: Gram staining a differential staining procedure which stains the gram positive cells with primary…

Q: Define the term gram staining.

A: Bacteria are a diverse group of microorganisms with a wide range of shapes, sizes, structures, and…

Q: This part of the staining process helps the primary stain to remain in the cell during…

A: Staining of biological samples is a very useful process for detailed observation and measurement of…

Q: Contrast the overall cell wall compositions of Gram positive and Gram negative bacteria, and go…

A: The bacteria are classified according to their cell wall composition into Gram positive and Gram…

Q: After performing the gram stain you observe your result as gram negative. To help confirm this…

A: Gram staining is a technique to identify bacterial stain gram-positive or gram-negative. It can be…

Q: what effect would failing to drain the smear in-between staining have on your judgment of the gram…

A: Gram staining is a technique used to differentiate between two groups of bacteria namely…

Q: It is expected that amoxicillin would be more effective against Gram-positive bacteria.  Explain…

A: Amoxicillin is a beta-lactam antibiotic that functions by attaching itself to the membrane proteins…

Q: What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What…

A: In microbiology, the technique that is used to enhance the contrast in samples, at the microscopic…

Q: Principle/ technique used in Flagella Stain Method/ Preparation/ Procedure of Flagella Stain

A: Flagella are narrow, slender whip-like structures made by flagellin protein that emerges from the…

Q: What type of stain is the Gram stain and what does it rely on for meaningful results

A:

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.