What are the structural differences between Gram-positive and Gram-negative bacteria cell walls? Can you explain what would happen to the cell wall's physical integrity while applying the Gram staining technique?
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What are the structural differences between Gram-positive and Gram-negative bacteria cell walls? Can you explain what would happen to the cell wall's physical integrity while applying the
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- You are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the alcohol. What is the appearance of the bacteria at this point? ○ pink ○ colorless ○ blue ○ purpleJanet is doing a Gram stain on a mixed culture that is known to contain both Gram positive and Gram negative bacteria. After finishing, she realizes she mixed up the safranin and alcohol steps. What is likely to be observed when she views the slide under the microscope? All cells will appear purple, regardless of whether they are actually Gram negative or Gram positive. All cells will appear pink, regardless of whether they are actually Gram negative or Gram positive. Gram negative cells will be clear, Gram positive cells will be purple. Gram positive cells will be clear, Gram negative cells will be pink. Cells will appear clear, regardless of whether they are actually Gram negative or Gram positive.Endospores can be seen as refractile structures in unstained cells and as colorless areas in Gram-stained cells. Why is it necessary to do an endospore stain to verify the presence of endospores?
- When performing a Gram stain on a Gram-negative bacteria, why does the bacteria stain pink? Crystal violet stain binds to iodine and remains in the bacterial cell wall because of the thick peptidoglycan layer. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. The alcohol wash removes crystal violet and iodine from the bacteria. Crystal violet stain binds to iodine and remains in the bacteria cell wall because of the thick peptidoglycan layer AND Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink AND The alcohol wash removes crystal violet and iodine from the bacteria.1)What is the natural color of cytoplasm? How is it related to our need to stain bacterial cells? 2) Which of the following is the main goal when staining a culture? A) improve contrast B) identify bacteria C) Visualize bacterial structuresYou use gram staining procedure to stain a bacterium that lacks a cell wall. What color will the bacterium be after staining procedure is finished?
- When you interpret a Gram-stained smear, you should also describe the morphology (shape) of the cells, and their arrangement. In the figure below, there are two distinct types of bacteria, distinguishable by Gram stain reaction, and also by their shape and arrangement. Below, describe these characteristics for both bacteria: Gram positive bacterium Gram negative bacterium Morphology cocci bacillus ArrangementThe bacterial cells in the image below were stained with a high concentration of carbolfuschin for an extended period of time (5 minutes) while the slide was heated. The slide was decolorized with a mixture of acid and alcohol. The slide was then counter-stained with methylene blue. What kind of stain does this describe?What benefits do endospores provide the cells during staining procedures?
- What color are Gram negative cells after decolorizing with ethanol? What is the best age for your culture when performing a Gram stain? Why? Why is Gram staining considered a differential staining process? What are the primary and counterstain? What is the purpose for using a counterstain? What are some reasons a Gram positive cell might appear Gram negative? What color would a Gram negative cell be if you forgot to wash with ethanol and why? What color would a Gram negative cell be if you forgot to stain it with safranin and why?Are there any chemical differences between the cell walls of gram-positive and negative bacteria that might explain differences in the rate of decolorization ? Does the age of a culture affect the gram stain reaction ? Why or why not?Explain the difference between Gram positive and Gram negative cell walls. Describe the procedure for performing a Gram stain. What is the procedure? How does each component work? (ie. What does the mordant do?) How is the Gram stain used clinically to help identify the cause of an infection?