What is the process to perform a Gram Stain? What happens if you make a mistake on a step (Think about different mistakes you could make)? What are the proper results? What structures do Archaeans use to attach to surfaces (Look like treble hooks)?What are bacterial inclusions?What are the similarities and differences in Gram - cells and Gram + cell structure?What is the function of ribosomes and what are they mad up of? What component of bacterial cells helps to combat/regulate osmotic forces? What are Koch’s postulates? What are they used for? Explain the process of endospore formation in endospore-producing organisms.What are the major components of the bacterial & eukaryotic cell?What is/are a pilus/pili and what do microbes use them for?What is the prokaryotic flagellum made up of?

Answered: What is the process to perform a Gram…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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What is the process to perform a Gram Stain? What happens if you make a mistake on a step (Think about different mistakes you could make)? What are the proper results?
What structures do Archaeans use to attach to surfaces (Look like treble hooks)?
What are bacterial inclusions?
What are the similarities and differences in Gram - cells and Gram + cell structure?
What is the function of ribosomes and what are they mad up of?
What component of bacterial cells helps to combat/regulate osmotic forces?
What are Koch’s postulates? What are they used for?
Explain the process of endospore formation in endospore-producing organisms.

What are the major components of the bacterial & eukaryotic cell?
What is/are a pilus/pili and what do microbes use them for?
What is the prokaryotic flagellum made up of?

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Question 1.

Gram stain is the method of staining used to differentiate and classify bacterial species into gram-positive and gram-negative. Gram-positive have a thick layer of peptidoglycan in the cell wall and no outer layer of lipids that retain the purple color of the primary stain (Crystal violet) after the addition of the counter stain (safranin). whereas, Gram-negative bacteria have a thin layer of peptidoglycan and an outer layer of lipids that don't absorb the primary stain and are washed out after adding safranin and show pink color.
Observations:
Pink color: gram-negative
Purple color: gram-positive

The mistakes such as over-staining or under-staining can cause Gram stains to decolorize too quickly will indicate falsely Gram-negative bacteria or too slowly will falsely indicate Gram-positive bacteria.

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