1. You were given a mixed nutrient agar broth culture of bacteria.  a. How will you determine the different types of bacteria present in the mixed culture? b. How will you make a pure culture of these bacteria in a slant nutrient agar? c. How will you identify these bacteria?

Q: There are two bacteria, and I have to find out the organism. I did gram staining, and I found out…

A: Gram staining is a laboratory technique used to differentiate and classify bacteria into two major…

Q: What is the purpose of flaming an inoculating loop?  How will you know when you have flamed the loop…

A: Hi, since you have posted multiple questions, we will be providing you with answers for the first…

Q: 1. A plate with a final dilution factor of 107 produced 210 colonies. a) What was the original…

A: Dilution is the process of diluting or mixing two or more substances or compounds; it is also known…

Q: What are the 2 most important reasons for using a positive control in Microbiology tests? What are…

A: A control test is used to set a reference for comparison in microbiological tests. It helps the…

Q: While working with Mannitol Salt Agar you notice the growth of staphylococci however other…

A: Mannitol Salt Agar (MSA) is a media which has high concentration of salt solution. There are found…

Q: he image below is the results of the Nutrient agar plate inoculated with the kitchen ounter sample…

A: Colony morphology is used by microbiologists to identify and characterized the different bacterial…

Q: The following media are used to grow bacteria for various purposes. Match the examples of media that…

A: MacConkey Agar: - Selective Growth Medium Malt Extract Agar:- Complex Medium

Q: Describe how you would execute this first stage from the point you are handed the nutrient broth…

A: For isolation of any culture, it needs to be incubated in broth to obtain full fledged turbidity or…

Q: What is it called when you stab the nutrient agar during the inoculation step of isolating a…

A: Introduction :  A solid or liquid media that contains nutrients and is used to cultivate bacteria or…

Q: ou've been tasked with observing the motility of an UNKNOWN Bacteria isolated from a mixed culture.…

A: Bacteria is the tiny cellular living structure which belong to the  Moneran kingdom. The bacteria…

Q: 1) What are all of the different colony types that can be seen on a mixed-culture streak plate?  2)…

A: Hi! Thanks for your question. As you have posted multiple questions, I am answering only the first…

Q: while working with mannitol salt agar you notice the growth of staphylococci however other…

A: Mannitol Salt Agar is used as a selective and differential medium for the identification and…

Q: Assume that you are adding 300 microliters of 1% substrate solution per well in a 24-well plate. If…

A: Fibronectin is a glycoprotein found in the extracellular matrix of cells and is also obtained from…

Q: In a Kligler Iron Agar/Triple Sugar Iron test you end up with a result with a yellow butt and red…

A: When a non-lactose fermenting bacterium hydrolyses the agar slant in the Kliger Iron Agar/ Triple…

Q: Can you please discuss the principles of using Motility Test Medium for the detection of bacterial…

A: Bacterial motility is usually demonstrated in a semi-solid agar medium. Motile bacteria ‘swarm' in…

Q: Which type(s) of medium would be used in each scenario?a. isolating the growth of Streptococcus…

A: A culture medium, also called the growth medium, can be a liquid or solid medium that supports the…

Q: Describe how each of the following would appear when grown on EMB agar: a. Escherichia coli b.…

A: EMB stands for Eosin Methylene Blue. This media is used as a seletive stain for the gram negative…

Q: A broth culture is initially inoculated with a single bacterial cell. For the first hour the…

A: * Given that  The bacteria has generation time of 30 minutes. So it takes about thirty minutes to…

Q: You decide to sample the bacteria growing on several items in a laboratory space. You follow aseptic…

A: By using an aseptic technique such as using applicator swabs dipped in a sterile broth solution to…

Q: Can you give me an example of a specific medium in culture media and fill the information below.…

A: Selective media are those that prevent the growth of undesirable microorganisms while promoting the…

Q: Indicate which of the following observations can be made when observing growth on nutrient agar.…

A: Colony morphology can be referred to as the visual appearance of bacterial colonies on a plate of…

Q: Refer to the provided image drawn by a student trying to plan out their serial dilution protocol.…

A: Colony forming unit (CFU)is a measure of viable bacterial cell. CFU/ml can be calculated using…

Q: A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution…

A: A sample of your blood, urine, skin, or any part of your body will be taken during a bacteria…

Q: After streaking microbial culture on agar plates and observing colonial growth, TMTC usually…

A: TMTC ("Too Many to Count") is a condition in which the number of colonies on an agar plate exceeds…

Q: Why do you think the continuous culture has higher chances of contamination than batch and fed-batch…

A: Continuous culture may be a set of techniques wont to reproducibly cultivate microorganisms at…

Q: 1. The differential aspect of MacConkey agar allows for the determination of which bacteria are…

A: selective media allow a certain type of bacteria to grow while inhibits the growth of other…

Q: - What is a pure culture? - Describe the procedure for making a streak plate from a clinical…

A: Introduction A growth media, also known as a culture medium, is a solid, liquid, or semi-solid…

Q: The statement, “In the laboratory, a sterile inoculating loop is moved across the agar surface in a…

A: The technique is streak plate technique. Streak plate method is a pure culture technique which helps…

Q: On which of the following types of media would you expect untransformed bacteria to grow on?

A: In this question, we have to answer which kind media support the growth of untransformed bacteria.

Q: A sample of Earth Alive Soil Activator was diluted by transferring 1 gram into a 9 ml dilution blank…

A: Microorganism concentrations are calculated using serial dilutions. A viable bacterial or fungal…

Q: Which of the following medium would be most appropriate for the bacterial cultures from stomach? a.…

A: Microorganism culture out of their natural environment in pure form in the laboratory has been…

Q: acillus cereus bacteria, in staining reaction tests, such as A.simple staining B.gram staining C.…

A: Answer

Q: You mixed up the numbers on the tubes when you inoculated the mannitol salt agar (MSA) plate. You do…

A: Differential media is a type of growth media used in the labs to grow and differentiate or…

Q: You have 2 mL of bacterial culture. How would you make a 10^-2 dilution using the entire culture…

A: The objective of this question is to understand how to make a 10^-2 dilution of a bacterial culture…

Q: You are working through the Gram Staining procedure on a mixed culture of S. aureus (G+) and E. coli…

A: During the Gram staining procedure the crystal violet dye is applied to the bacterial cells followed…

Q: you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each…

A: In the study of microorganisms, a mixed culture is one that contains more than one type of organism…

Q: You are interested in the total bacterial load of the bat guano. In order to determine this, you go…

A: For calculation of total bacterial count we choose the solution that usually gives the colony number…

Q: Define what is a minimal medium and the nature of its components.

A: A culture medium can be defined as solid,liquid or semi solid medium which helps in support of the…

Q: you are given a mix culture of S aureus, E. coli and P. aeruginosa. besides using the streak plate…

A: The first requirement for physically isolating a bacterium is that it can be cultured in the…

Q: Which isolation technique is best used in isolating microorganisms (in countable good plates) from a…

A: The phrase "isolation" in microbiology refers to the separation of a strain from a natural, mixed…

Q: What type of medium is best used to maintain a preserve specimen before clinical analysis? A.…

A: A growth or culture medium is solid, liquid or semi solid in nature that is used to support the…

Q: Summarize briefly the pasteurization step. Why is there a need to pasteurize the milk for at least…

A: Note: As Per Guidelines, We Can Answer One Question At A Time. Ask Again To get rest answers.…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.