What is the purpose of adding mineral oil to an O/F Media tube and why do we only put mineral oil in only one?

Q: Which of the following is the basic difference between serological (blow-out) and measuring…

A: A pipette is a sort of laboratory instrument that is frequently used in biology and chemistry to…

Q: What is the purpose of sealing the flask with a balloon? What product is captured in the balloon?

A: Fermentation is the process of ATP generation by the microorganism. Carbohydrates are the substrate…

Q: what does chroomatography observation mean

A: Column fractionation was developed in the 1890s as a technique of separating the various components…

Q: A culture is incubated for 10 hours. 1 hours after inoculation it reached the exponential growth…

A: This question involves the calculation of the growth constant (k) and generation time (g(t)) for a…

Q: why is the tube passed through the flame after removing and before replacing the cap

A: It is a aseptic technique follows during the inoculation of pure culture of microorganism. This…

Q: In using an ultracentrifuge, arrange the following steps in correct order A. Turn the knob to the…

A: Centrifugation is a techniques in which involves centrifugal force to separate the particles from…

Q: What is this test? What coated the bottom of the wells? What were we testing for? How did we know it…

A: The test is ELISA. It is an Enzyme linked Immunosorbant Assay, which is used in many applications.…

Q: What is the result of tube A in this Voges-proskauer test? What are the reagents of this test? What…

A: Voges-proskauer test is performed to differentiate between facultative anaerobic enteric bacteria.

Q: explain why it is not good to break sterile field  when inserting a foley catheter

A: Inserting the indwelling catheter is an invasive procedure. Sterile technique should follow while…

Q: What are some methods to prevent contamination during streak plating a medium?

A: Streak plate method is one of the most important methods used by the laboratories to isolate single…

Q: Why Purified synthetic alcohol is preferred for level vial?

A: An alcohol is an organic compound that carries at least one hydroxyl functional group (-OH) bound to…

Q: Give two ways to prevent the diffusion of oxygen into the medium in this test.

A: The main two ways to prevent the diffusion of oxygen are given below.

Q: What is the difference between the pour plate method and the spread plate method? Why might you…

A: Standard plate count method is a growth based approach that counts the number of living…

Q: Why is it important to eject a small amount of blood from the syringe sample onto a gauze pad before…

A: Blood gas analysis is a diagnostic test. It gives useful information regarding the respiratory and…

Q: Why is there a need to filter the alkaline solution after adding the Barium chloride solution?

A: In an aqueous solution, barium chloride acts as a simple salt.  However when dissolved in water, it…

Q: explain why it is important to maintain sterility when inserting a foley cathether

A: Foley's catheter is an indwelling catheter, i.e. it can be left in place for longer durations than…

Q: What are the advantages and disadvantages of the filter method in the analysis of water samples?

A: Utilising the membrane filter method, test is gone through the layer utilising a filter funnel and…

Q: a)Determine the amount of X (in gram) in order to prepare 100 mL of 50 mM stock solution of X. Show…

A: In this question, we are given: a) Molecular weight of X= 225 Molarity of stock solution= 50 mM…

Q: What is the difference between normal and reverse phase chromatography?

A: The main Difference between Normal and reverse phase chromatography is 1) In normal phase…

Q: Why are the tubes sealed with a rubber stopper rather than left open to the atmosphere?

A: A rubber stopper is used to seal the tube opening as well as other laboratory glassware such as…

Q: what is the purpose of the FeCl3 in this aspirin test?

A: Now, setting up and observing the breakdown:1) Divided: We split apirin into two tubes, one with HCl…

Q: In step 3 of today's protocol, it mentions that the incubation step with the P2 buffer should not be…

A: P2 buffer is a lysis buffer solution containing sodium dodecyl sulfate to puncture holes in cellular…

Q: Which of the following is the a-anomer? H но H Но H2C H ОН H2C- нн ОН со 4 1 2 1 Submit 3 -OH Н ОН…

A: Alpha anomer

Q: Explain why the following step are necessary during subculturing:  Flaming the neck of…

A: Aseptic technique-Aseptic technique should be observed whenever microorganisms are transferred from…

Q: Why is UV light used in Column chromatography experiment ?

A: A chromatography procedure used to separate a single chemical compound from a mixture is known as…

Q: chemical(s) best at inhibiting both Gram- negative and Gram-positive bacteria [ Choose ] chemical…

A: Bacteria are microscopic, unicellular, prokaryotic organisms found in diverse environments. They can…

Q: the step by step process of Nitrate Redution Test using a schematic diagram

A: Nitrate reduction test This test is widely used in microbiology laboratories to determine the…

Q: Which is more accurate a transfer pipette or a measuring pipette? How are these compared to a…

A: Features Transfer pipette Measuring pipette MicropipettePurposeUsed for simple liquid transfers…

Q: What is the principle of counting with a hemocytometer? What are the advantages and disadvantages?

A: Answer: HEMOCYTOMETER : It is the tool used in most of the biochemistry and biology labs in order to…

Q: How to use the sterile filter? Show the different sizes and specifications of sterile filters.

A: Sterilization is the method that is used to eliminate all forms of life and biological agents…

Q: Of the two principal polymers used in column chromatography and electrophoresis, which one would be…

A: Column chromatography is a purification method that uses the polarity of compounds for the…

Q: Prepare the following LB media with antibiotic added. A 100ml of LB media with 25 micro/ml of Amp…

A: Luria-Bertani (LB) broth is a liquid media comprised of several carbon and nitrogen sources. It is…

Q: You are testing a sample of water from a swimming pool. You count a plate made from a 1 mL transfer…

A: Dilution is a process by which solution (water or diluents) are added to the solvent (main solution)…

Q: Which of the following statements is correct about column chromatography? a) Resolution improves as…

A: Column chromatography is an isolation technique used to separate different components in a solution.…

Q: What is this instrument calledi

A: A microbiology laboratory is one that specialises in the cultivation, analysis, and identification…

Q: Explain how to conduct a Kirby-Bauer disk diffusion test. Include all of the steps involved

A: INTRODUCTION Kirby - Bauer Disc diffusion method Kirby - bauer disc diffusion method is widely known…

Q: Distance of color diameter (mm) 20 15 KMNO, 0.1M - KMNO, 1.0M - 5. 15 45 09 75 Time (minutes) 06

A: * The molecules movement in  agarose gel is dependent on the  size  charge  *And pore size also…

Q: Are TSA, MRS and MOX differential media? Why or why not?

A: In microbial biology, differential media refers to any growth media that comprises of certain…

Inheritance and Mendelian Genetics

Inheritance means the passing of traits to offspring from parents. These traits could be passed either through asexual reproduction or sexual reproduction. The offspring receives the genetic material from the parents. Mendelian inheritance is a certain biological inheritance that follows the rules and principles laid down by Gregor Mendel in 1865. The initial hypothesis was considered wrong, but other inheritance theories modified the concept of inheritance and thus genetics.