In step 3 of today's protocol, it mentions that the incubation step with the P2 buffer should not be allowed to proceed for more than 5 minutes. a) What is the chemical composition and concentration (not mass) of each component of the P2 buffer?b) c) What is the purpose of adding the P2 buffer? What would be the major problem if this step is allowed to proceed for a long period of time?

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