thanol has been carried over into your DNA sample (two answers possible here, select both of them) a) The column tip touched the column flow through b) The cells stayed in the lysis buffer for too long c) You didn't add enough wash buffer d) You did not centrifuge a final time after the final wash

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Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.